Cancer Immunology, Immunotherapy

, 60:1609

Poly(I:C)-induced tumour cell death leads to DC maturation and Th1 activation

  • Edit Kovalcsik
  • Katie Lowe
  • Mike Fischer
  • Angus Dalgleish
  • Mark D. Bodman-Smith
Original article

DOI: 10.1007/s00262-011-1058-7

Cite this article as:
Kovalcsik, E., Lowe, K., Fischer, M. et al. Cancer Immunol Immunother (2011) 60: 1609. doi:10.1007/s00262-011-1058-7

Abstract

Dendritic cells (DCs) have the ability to generate peptide epitopes for MHC class I molecules derived from apoptotic tumour cells for direct recognition by cytotoxic T cells. This function has lead to DCs being used in vaccine strategies. In this study, we investigate the effect of inducing apoptosis in tumour cell lines using IFN-γ and poly(I:C), the subsequent maturation of the endocytosing DC and its ability to direct the resulting T cell response. We show that uptake of poly(I:C)-induced apoptotic tumour cells leads to DC maturation and activation with a Th1 cell polarising capacity. In contrast, these effects are not seen by DCs loaded with γ-irradiated apoptotic tumour cells. We propose that the manner in which tumour cells are induced to die can have a profound effect on the endocytosing DC and the resulting T cell response.

Keywords

Dendritic cellsTumour cellsApoptosisPoly(I:C)T helper cell type 1

Abbreviations

IFN-γ

Interferon-gamma

dsRNA

Double stranded RNA

IFN-γ/poly(I:C) tumour cell

IFN-γ pre-treated and poly(I:C) pulsed or loaded tumour cell

Supplementary material

262_2011_1058_MOESM1_ESM.pdf (536 kb)
Supplementary material 1 (PDF 536 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Edit Kovalcsik
    • 1
  • Katie Lowe
    • 1
  • Mike Fischer
    • 1
  • Angus Dalgleish
    • 1
  • Mark D. Bodman-Smith
    • 1
  1. 1.Systems Immunology Group, Cellular and Molecular MedicineSt George’s University of LondonLondonUK