Cancer Immunology, Immunotherapy

, Volume 60, Issue 6, pp 869–882

Leukocyte infiltrate in gastrointestinal adenocarcinomas is strongly associated with tumor microsatellite instability but not with tumor immunogenicity

  • Mónica Bernal
  • Angel Concha
  • Pablo Sáenz-López
  • Ana Isabel Rodríguez
  • Teresa Cabrera
  • Federico Garrido
  • Francisco Ruiz-Cabello
Original Article

DOI: 10.1007/s00262-011-0999-1

Cite this article as:
Bernal, M., Concha, A., Sáenz-López, P. et al. Cancer Immunol Immunother (2011) 60: 869. doi:10.1007/s00262-011-0999-1

Abstract

Purpose

To analyze the correlation of genomic instability with leukocyte infiltrate in gastrointestinal carcinomas (GIACs) and with tumor immunogenicity, e.g., HLA class I cell surface expression defects and galectin-3 and PDL-1 expression.

Experimental design

Lymphocyte and macrophage infiltrations were immunohistochemically studied in HLA class I negative GIACs with sporadic high-level microsatellite instability (MSI-H) or microsatellite stability (MSS).

Results

Tumors with MSI-H were associated with the following: dense infiltration (CD45, P < 0.001); cytotoxic CD8-positive lymphocytes (P < 0.001); and a complete absence of HLA class I cell surface expression, due to inactivating β2-microglobulin (β2-m) mutation in 50% of cases. In contrast, HLA class I negative tumors with MSS were significantly associated with fewer CD8-positive lymphocytes. There was no association between microsatellite instability and other molecular features of the tumor cells, including expression of galectin-3. Finally, macrophage infiltrate in the tumors was not correlated with microsatellite instability or HLA class I cell surface expression (CD64, P = 0.63; CD163, P = 0.51).

Conclusions

Microsatellite instability appears to be the most important factor determining the composition, density, and localization of leukocyte infiltrate, which is independent of other molecular features such expression of HLA class I cells, galectin-3, or programmed death ligand-1. Accordingly, the strong intratumoral CD8+ T infiltration of MSI-H tumors may be produced by elevated levels of specific inflammatory chemokines in the tumor microenvironment.

Keywords

Gastrointestinal adenocarcinomas (GIACs)Microsatellite instability (MSI)HLA class ITumor leukocyte infiltrate

Abbreviations

APM

Antigen processing machinery

CRC

Colorectal cancer

GIAC

Gastrointestinal adenocarcinoma

HLA

Human leukocyte antigen

LOH

Loss of heterozygosity

MHC

Major histocompatibility complex

MMR

Mismatch repair

MSI

Microsatellite instability

MSI-H

High microsatellite instability, MSS, microsatellite stability

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Mónica Bernal
    • 1
  • Angel Concha
    • 2
  • Pablo Sáenz-López
    • 1
  • Ana Isabel Rodríguez
    • 1
  • Teresa Cabrera
    • 1
    • 3
  • Federico Garrido
    • 1
    • 3
  • Francisco Ruiz-Cabello
    • 1
    • 3
  1. 1.Servicio de Análisis Clínicos e InmunologíaHospital Universitario Virgen de las NievesGranadaSpain
  2. 2.Servicio de Anatomía PatológicaHospital Universitario Virgen de las NievesGranadaSpain
  3. 3.Departamento de Bioquímica, Biología Molecular III e Inmunología, Facultad de MedicinaGranadaSpain