Cancer Immunology, Immunotherapy

, Volume 59, Issue 6, pp 885–897

Maitake beta-glucan promotes recovery of leukocytes and myeloid cell function in peripheral blood from paclitaxel hematotoxicity

  • Hong Lin
  • Elisa de Stanchina
  • Xi Kathy Zhou
  • Feng Hong
  • Andrew Seidman
  • Monica Fornier
  • Wei-Lie Xiao
  • Edward J. Kennelly
  • Kathleen Wesa
  • Barrie R. Cassileth
  • Susanna Cunningham-Rundles
Original Article

DOI: 10.1007/s00262-009-0815-3

Cite this article as:
Lin, H., de Stanchina, E., Zhou, X.K. et al. Cancer Immunol Immunother (2010) 59: 885. doi:10.1007/s00262-009-0815-3

Abstract

Bone marrow myelotoxicity is a major limitation of chemotherapy. While granulocyte colony stimulating factor (G-CSF) treatment is effective, alternative approaches to support hematopoietic recovery are sought. We previously found that a beta-glucan extract from maitake mushroom Grifola frondosa (MBG) enhanced colony forming unit-granulocyte monocyte (CFU-GM) activity of mouse bone marrow and human hematopoietic progenitor cells (HPC), stimulated G-CSF production and spared HPC from doxorubicin toxicity in vitro. This investigation assessed the effects of MBG on leukocyte recovery and granulocyte/monocyte function in vivo after dose intensive paclitaxel (Ptx) in a normal mouse. After a cumulative dose of Ptx (90–120 mg/kg) given to B6D2F1mice, daily oral MBG (4 or 6 mg/kg), intravenous G-CSF (80 µg/kg) or Ptx alone were compared for effects on the dynamics of leukocyte recovery in blood, CFU-GM activity in bone marrow and spleen, and granulocyte/monocyte production of reactive oxygen species (ROS). Leukocyte counts declined less in Ptx + MBG mice compared to Ptx-alone (p = 0.024) or Ptx + G-CSF treatment (p = 0.031). Lymphocyte levels were higher after Ptx + MBG but not Ptx + G-CSF treatment compared to Ptx alone (p < 0.01). MBG increased CFU-GM activity in bone marrow and spleen (p < 0.001, p = 0.002) 2 days after Ptx. After two additional days (Ptx post-day 4), MBG restored granulocyte/monocyte ROS response to normal levels compared to Ptx-alone and increased ROS response compared to Ptx-alone or Ptx + G-CSF (p < 0.01, both). The studies indicate that oral MBG promoted maturation of HPC to become functionally active myeloid cells and enhanced peripheral blood leukocyte recovery after chemotoxic bone marrow injury.

Keywords

ChemotherapyHematotoxicityPaclitaxelBeta-glucanBone marrow and leukocyte recoveryHematopoietic progenitor cells

Supplementary material

262_2009_815_MOESM1_ESM.doc (71 kb)
Supplementary material (DOC 71KB)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Hong Lin
    • 1
  • Elisa de Stanchina
    • 2
  • Xi Kathy Zhou
    • 3
  • Feng Hong
    • 4
  • Andrew Seidman
    • 5
  • Monica Fornier
    • 5
  • Wei-Lie Xiao
    • 6
  • Edward J. Kennelly
    • 6
  • Kathleen Wesa
    • 4
  • Barrie R. Cassileth
    • 4
  • Susanna Cunningham-Rundles
    • 1
  1. 1.Cellular Immunology Laboratory, Division of Hematology/Oncology, Department of PediatricsWeill Medical College of Cornell UniversityNew YorkUSA
  2. 2.Antitumor Assessment Core FacilityMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  3. 3.Division of Biostatistics and Epidemiology, Department of Public HealthWeill Medical College of Cornell UniversityNew YorkUSA
  4. 4.Integrative Medicine ServiceMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  5. 5.Breast Cancer Medicine Service, Department of MedicineMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  6. 6.Department of Biological SciencesLehman College, City University of New YorkNew YorkUSA