Cancer Immunology, Immunotherapy

, Volume 56, Issue 11, pp 1845–1852

STAT1-dependent and STAT1-independent gene expression in murine immune cells following stimulation with interferon-alpha

Authors

  • Jason M. Zimmerer
    • Integrated Biological Sciences Graduate ProgramThe Ohio State University Comprehensive Cancer Center
    • Human Cancer Genetics Program Department of Molecular Virology, Immunology, and Medical GeneticsThe Ohio State University Comprehensive Cancer Center
  • Gregory B. Lesinski
    • Human Cancer Genetics Program Department of Molecular Virology, Immunology, and Medical GeneticsThe Ohio State University Comprehensive Cancer Center
  • Michael D. Radmacher
    • Center for BiostastisticsThe Ohio State University Comprehensive Cancer Center
  • Amy Ruppert
    • Center for BiostastisticsThe Ohio State University Comprehensive Cancer Center
    • Human Cancer Genetics Program Department of Molecular Virology, Immunology, and Medical GeneticsThe Ohio State University Comprehensive Cancer Center
    • Division of Surgical Oncology, Department of SurgeryThe Ohio State University Comprehensive Cancer Center
Original Article

DOI: 10.1007/s00262-007-0329-9

Cite this article as:
Zimmerer, J.M., Lesinski, G.B., Radmacher, M.D. et al. Cancer Immunol Immunother (2007) 56: 1845. doi:10.1007/s00262-007-0329-9

Abstract

Purpose

The precise molecular targets of interferon-alpha (IFN-α) therapy of melanoma are unknown but likely involve signal transducer and activator of transcription 1 (STAT1) signal transduction within host immune effector cells. We hypothesized that microarray analysis could be utilized to identify candidate molecular targets important for mediating the anti-tumor effect of exogenously administered IFN-α.

Experimental Methods

To identify the STAT1-dependent genes regulated by IFN-α, the gene expression profile of splenocytes from wild type (WT) and STAT1−/− mice was characterized.

Results

This analysis identified 30 genes that required STAT1 signal transduction for optimal expression in response to IFN-α (p < 0.001). These genes include granzyme b (Gzmb), interferon regulatory factor 7 (Irf7), Fas death domain-associated protein (Daxx), and lymphocyte antigen 6 complex, locus C (Ly6c). The expression of 20 genes was found to be suppressed in the presence of STAT1 including chemokine ligand 2 (Ccl2), Ccl5, and Ccl7. Nineteen genes were significantly upregulated in murine splenocytes following treatment with IFN-α regardless of the presence of STAT1 including CD86, lymphocyte antigen 6 complex, locus A (Ly6a), and Tap binding protein (Tapbp). The expression of representative IFN-responsive genes was confirmed at the transcriptional level by Real Time PCR.

Conclusion

This report is the first to demonstrate that STAT1-mediated signal transduction plays a major role in the transcriptional response of murine immune cells to IFNα.

Keywords

Interferon-alphaOligonucleotide microarray analysisSTAT1Immune cells

Copyright information

© Springer-Verlag 2007