Cancer Immunology, Immunotherapy

, Volume 56, Issue 8, pp 1251–1264

Encapsulation in liposomal nanoparticles enhances the immunostimulatory, adjuvant and anti-tumor activity of subcutaneously administered CpG ODN

  • Susan de Jong
  • Ghania Chikh
  • Laura Sekirov
  • Sam Raney
  • Sean Semple
  • Sandra Klimuk
  • Ning Yuan
  • Micheal Hope
  • Pieter Cullis
  • Ying Tam
Original Article

DOI: 10.1007/s00262-006-0276-x

Cite this article as:
de Jong, S., Chikh, G., Sekirov, L. et al. Cancer Immunol Immunother (2007) 56: 1251. doi:10.1007/s00262-006-0276-x

Abstract

Immunostimulatory oligodeoxynucleotides (ODN) containing cytosine-guanine (CpG) motifs are powerful stimulators of innate as well as adaptive immune responses, exerting their activity through triggering of the Toll-like receptor 9. We have previously shown that encapsulation in liposomal nanoparticles (LN) enhances the immunostimulatory activity of CpG ODN (LN-CpG ODN) (Mui et al. in J Pharmacol Exp Ther 298:1185, 2001). In this work we investigate the effect of encapsulation on the immunopotency of subcutaneously (s.c.) administered CpG ODN with regard to activation of innate immune cells as well as its ability to act as a vaccine adjuvant with tumor-associated antigens (TAAs) to induce antigen (Ag)-specific, adaptive responses and anti-tumor activity in murine models. It is shown that encapsulation specifically targets CpG ODN for uptake by immune cells. This may provide the basis, at least in part, for the significantly enhanced immunostimulatory activity of LN-CpG ODN, inducing potent innate (as judged by immune cell activation and plasma cytokine/chemokine levels) and adaptive, Ag-specific (as judged by MHC tetramer positive T lymphocytes, IFN-γ secretion and cytotoxicity) immune responses. Finally, in efficacy studies, it is shown that liposomal encapsulation enhances the ability of CpG ODN to adjuvanate adaptive immune responses against co-administered TAAs after s.c. immunization, inducing effective anti-tumor activity against both model and syngeneic tumor Ags in murine tumor models of thymoma and melanoma.

Keywords

CpG Liposome Nanoparticle Immunostimulatory Anti-tumor Vaccine 

Abbreviations

APC

Antigen presenting cells

LN

Liposomal nanoparticles

CpG

Unmethylated CpG dinucleotides

ODNs

Oligodeoxynucleotides

TRP-2

Tyrosinase-related protein 2

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Susan de Jong
    • 1
  • Ghania Chikh
    • 2
  • Laura Sekirov
    • 2
  • Sam Raney
    • 2
  • Sean Semple
    • 2
  • Sandra Klimuk
    • 2
  • Ning Yuan
    • 2
  • Micheal Hope
    • 2
  • Pieter Cullis
    • 1
  • Ying Tam
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of British ColumbiaVancouverCanada
  2. 2.Inex Pharmaceuticals CorporationBurnabyCanada