Original Article

Cancer Immunology, Immunotherapy

, Volume 56, Issue 7, pp 1087-1096

MIP3 alpha stimulates the migration of Langerhans cells in models of human papillomavirus (HPV)-associated (pre)neoplastic epithelium

  • Ludivine HermanAffiliated withDepartment of Pathology, CRCE, B35, University of Liege
  • , Pascale HubertAffiliated withDepartment of Pathology, CRCE, B35, University of Liege
  • , Jean-Hubert CabergAffiliated withDepartment of Pathology, CRCE, B35, University of Liege
  • , Brigitte EvrardAffiliated withDepartment of Pharmaceutical Technology, CRCE, B35, University of Liege
  • , Witold KedziaAffiliated withDepartment of Gynecology and Obstetrics, Karol Marcinkowski School of Medecine
  • , Jacques BoniverAffiliated withDepartment of Pathology, CRCE, B35, University of Liege
  • , Philippe DelvenneAffiliated withDepartment of Pathology, CRCE, B35, University of Liege Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Although human papillomavirus (HPV) DNA is detected in the majority of cervical cancers and their precursors (squamous intraepithelial lesions; SIL), the persistence or progression of cervical lesions could be associated with quantitative and functional alterations of dendritic/Langerhans cells (DC/LC). As LC abnormalities have been associated with a decreased expression of macrophage inflammatory protein 3α (MIP3α) in cervical SIL, we tested the effect of exogenous MIP3α on the migration of LC in a (pre)neoplastic epithelium formed in vitro. By using a Boyden chamber assay, we first showed that the migratory capacity of LC generated in vitro is significantly increased in the presence of MIP3α compared to control medium. We next demonstrated that MIP3α is able to increase the 3D infiltration of LC in organotypic cultures of HPV-transformed keratinocytes. This property to stimulate LC migration was not altered after inclusion of MIP3α in a bioadhesive polycarbophil gel. Moreover, the function of DC to exert cytostatic effects and to present alloantigens was not altered in the presence of MIP3α.

Keywords

Langerhans cells MIP3 alpha Human papillomavirus Uterine cervix Organotypic culture Cell migration