Review

Cancer Immunology, Immunotherapy

, Volume 53, Issue 3, pp 139-143

First online:

Identification of tumour antigens by serological analysis of cDNA expression cloning

  • Geng LiAffiliated withSchool of Science, The Nottingham Trent University
  • , Amanda MilesAffiliated withSchool of Science, The Nottingham Trent University
  • , Aija LineAffiliated withBiomedical Research and Study Centre, University of Latvia
  • , Robert C ReesAffiliated withSchool of Science, The Nottingham Trent University Email author 

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Abstract

The identification of antigens that distinguish normal cells from cancer cells is an important challenge in the field of tumour immunology and immunotherapy. The immunoscreening of cDNA expression libraries constructed from human tumour tissues with antibodies in sera from cancer patents (SEREX: serological identification of antigens by recombinant expression cloning) provides a powerful approach to identify immunogenic tumour antigens. To date, over 2,000 tumour antigens have been identified from a variety of malignancies using SEREX. These antigens can be classified into several categories, of which the cancer/testis (CT) antigens appear to be the most attractive candidates for vaccine development. The SEREX-defined tumour antigens facilitate the identification of epitopes (antigenic peptides) recognised by antigen-specific cytotoxic T lymphocytes (CTLs) and provide a basis for peptide vaccine and gene therapy in a wide variety of human cancers. Moreover, some of these antigens seem to play a functional role in the pathogenesis of cancer.

Keywords

Tumour antigen cDNA cloning Serological analysis Antigen identification