Cancer Immunology, Immunotherapy

, Volume 52, Issue 10, pp 643–647

Generation of non-permissive basement membranes by anti-laminin antibody fragments produced by matrix-embedded gene-modified cells

Authors

  • Laura Sanz
    • Department of ImmunologyHospital Universitario Clínica Puerta de Hierro
  • Mónica Feijóo
    • Department of ImmunologyHospital Universitario Clínica Puerta de Hierro
  • Belén Blanco
    • Department of ImmunologyHospital Universitario Clínica Puerta de Hierro
  • Antonio Serrano
    • Department of Immunology and OncologyCentro Nacional de Biotecnología
    • Department of ImmunologyHospital Universitario Clínica Puerta de Hierro
Original Article

DOI: 10.1007/s00262-003-0400-0

Cite this article as:
Sanz, L., Feijóo, M., Blanco, B. et al. Cancer Immunol Immunother (2003) 52: 643. doi:10.1007/s00262-003-0400-0

Abstract

Tumor-induced blood vessel formation is a key process for the growth and spread of solid tumors, traditionally attributed to activated host endothelial cells (angiogenesis). Recently, highly aggressive cancer cells have been shown to form vascular channels in the absence of endothelial cells (vasculogenic mimicry). In this work, we have focused on the common dependence of both processes in their interactions with the surrounding extracellular matrix. We had previously described a human recombinant anti-laminin antibody that blocked the capillary morphogenesis of human endothelial cells. Here, we demonstrate that the purified antibody is capable of inhibiting channel formation by human cancer cells, suggesting a common morphogenic pathway in both processes. Moreover, matrix-embedded cells producing antibody fragments may render the surrounding matrix non-permissive for aggressive tumor cells. These results open the way for the development of new therapeutic strategies for cancer.

Keywords

Extracellular matrix Laminin Capillary morphogenesis Tumor plasticity Single-chain antibody fragments

Copyright information

© Springer-Verlag 2003