Cancer Immunology, Immunotherapy

, Volume 52, Issue 7, pp 423–428

Prognostic value of the CD4+/CD8+ ratio of tumour infiltrating lymphocytes in colorectal cancer and HLA-DR expression on tumour cells

  • Axel C. P. Diederichsen
  • J. v. B. Hjelmborg
  • Per B. Christensen
  • Jesper Zeuthen
  • Claus Fenger
Original Article

DOI: 10.1007/s00262-003-0388-5

Cite this article as:
Diederichsen, A.C.P., v. B. Hjelmborg, J., Christensen, P.B. et al. Cancer Immunol Immunother (2003) 52: 423. doi:10.1007/s00262-003-0388-5


The purpose of this study was to clarify whether HLA-DR expression of colorectal tumour cells or the CD4+/CD8+ ratio of the tumour infiltrating lymphocytes is significantly associated with the prognosis of colorectal cancer. Using flow cytometry, we studied the tumour cell expression of the HLA class II in 70 enzymatically dissociated colorectal cancers and the phenotype of tumour infiltrating lymphocytes (TILs) in 41 cases. There was no trend in 5-year survival between three levels (low, medium, high) of HLA-DR expression on the tumour cells. Patients with low CD4+/CD8+ ratios had a better clinical course, with significantly higher 5-year survival, p=0.046, independent of the Dukes stage and age. Our results have implications for tumour immunology; colorectal cancer cells might be a target for cytotoxic T-lymphocytes, however the tumour cells are not able to initiate an immune response. Stimulation of the immune system could possible be obtained using dendritic cells cultured in vitro and loaded with tumour antigens.


Survival rateColorectal cancerTumour infiltrating lymphocytesCD4+/CD8+ ratioHLA class II

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Axel C. P. Diederichsen
    • 1
  • J. v. B. Hjelmborg
    • 2
  • Per B. Christensen
    • 3
  • Jesper Zeuthen
    • 4
  • Claus Fenger
    • 5
  1. 1.Biomedical LaboratoryUniversity of Southern DenmarkOdense CDenmark
  2. 2.Institute of Public HealthResearch Unit of Epidemiology University of Southern DenmarkOdenseDenmark
  3. 3.Department of Surgical GastroenterologyFunen HospitalSvendborgDenmark
  4. 4.Department of Tumour Cell Biology Division of Cancer BiologyDanish Cancer SocietyDenmark
  5. 5.Department of PathologyOdense University HospitalOdenseDenmark