European Journal of Nuclear Medicine

, Volume 25, Issue 10, pp 1383–1389

Imaging of metastatic melanoma utilising a technetium-99m labelled RGD-containing synthetic peptide

Authors

  • Gregory B. Sivolapenko
    • ”Encephalos” Research and Therapeutic Institute, Athens, Greece
  • Demosthenis Skarlos
    • ”Agii Anargiri” Cancer Hospital, Athens, Greece
  • Demitris Pectasides
    • ”Metaxa” Cancer Hospital, Athens, Greece
  • Evaggelia Stathopoulou
    • ”Encephalos” Research and Therapeutic Institute, Athens, Greece
  • Alexandros Milonakis
    • ”Encephalos” Research and Therapeutic Institute, Athens, Greece
  • George Sirmalis
    • ”Encephalos” Research and Therapeutic Institute, Athens, Greece
  • Alan Stuttle
    • Antisoma plc., London, UK
  • Nigel S. Courtenay-Luck
    • Antisoma plc., London, UK
  • Konstantinos Konstantinides
    • ”Encephalos” Research and Therapeutic Institute, Athens, Greece
  • Agamemnon A. Epenetos
    • Antisoma plc., London, UK
Original article

DOI: 10.1007/s002590050312

Cite this article as:
Sivolapenko, G., Skarlos, D., Pectasides, D. et al. Eur J Nucl Med (1998) 25: 1383. doi:10.1007/s002590050312

Abstract.

Integrins are cell-surface glycoproteins found in different forms on all cells except erythrocytes. Integrins bind to cell adhesion molecules and to proteins found in the extracellular matrix. A tripeptidic sequence Arg-Gly-Asp (RGD) is often the primary site of recognition by integrins which are expressed on tumour cells and are responsible for tumour invasion and metastasis. A synthetic decapeptide designated αP2 containing two RGD sequences radiolabelled with technetium-99m was used to image malignant melanoma in vivo. Fourteen patients previously diagnosed with metastatic melanoma underwent gamma camera imaging 20–180 min following intravenous administration of the radiolabelled synthetic decapeptide αP2. Six out of eight (6/8) of the lymph node metastases (75%) and all other neoplastic sites (11 sites) were successfully imaged, with the exception of three sites in the mediastinal area which were not positively imaged. In two cases there was false positive uptake in the rounded pigmented areolar/nipple area. In three cases (seven sites) the peptide scan confirmed the absence of disease in suspected lesions (true-negative). The synthetic peptide was rapidly removed from the circulation by filtration through the kidneys and excretion in the urine. No toxicity or adverse events were recorded. Radiolabelled αP2 peptide, which binds specifically to adhesion molecules on tumours, can be used for the in vivo detection of neoplastic metastases.

Key words: IntegrinsSynthetic peptideMalignant melanomaScintigraphy

Copyright information

© Springer-Verlag Berlin Heidelberg 1998