Original article

European Journal of Nuclear Medicine and Molecular Imaging

, Volume 33, Issue 10, pp 1171-1177

First online:

Labelling of human mesenchymal stem cells with indium-111 for SPECT imaging: effect on cell proliferation and differentiation

  • L. BindslevAffiliated withStem Cell Laboratory 9312, The Heart Centre, Rigshospitalet, University of Copenhagen
  • , M. Haack-SørensenAffiliated withStem Cell Laboratory 9312, The Heart Centre, Rigshospitalet, University of Copenhagen
  • , K. BisgaardAffiliated withDepartment of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, University of CopenhagenCVU Oeresund, Faculty of Medical Laboratory Sciences,
  • , L. KraghAffiliated withDepartment of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, University of Copenhagen
  • , S. MortensenAffiliated withStem Cell Laboratory 9312, The Heart Centre, Rigshospitalet, University of Copenhagen
  • , B. HesseAffiliated withDepartment of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, University of CopenhagenCluster for Molecular Imaging, University of Copenhagen
  • , A. KjærAffiliated withDepartment of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, University of CopenhagenCluster for Molecular Imaging, University of Copenhagen
  • , J. KastrupAffiliated withStem Cell Laboratory 9312, The Heart Centre, Rigshospitalet, University of CopenhagenCardiac Catheterisation Laboratory 2014,The Heart Centre, Rigshospitalet Email author 

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Abstract

Purpose

Stem cell therapy seems to be a new treatment option within cardiac diseases to improve myocardial perfusion and function. However, the delivery and traceability of the cells represent a problem. Radioactive labelling with 111In could be a method for tracking mesenchymal stem cells (MSCs). However, 111In could influence the viability and differentiation capacity of MSCs, which would limit its use. Therefore, the aim of this study was to evaluate the influence of 111In labelling in doses relevant for SPECT imaging in humans on the viability and differentiation capacity of human MSCs.

Methods and results

Human MSCs isolated from bone marrow were incubated with 111In-tropolone (15–800 Bq/cell). The labelling efficiency was approximately 25% with 30 Bq/cell 111In. The MSC doubling time was 1.04±0.1 days and was not influenced by 111In within the range 15–260 Bq/cell. Using 30 Bq 111In/cell it was possible to label MSCs to a level relevant for clinical scintigraphic use. With this dose, 111In had no effect on characteristic surface and intracellular markers of cultured MSCs analysed both by flow cytometry and by real-time polymerase chain reaction. Further, the labelled MSCs differentiated towards endothelial cells and formed vascular structures.

Conclusion

It is possible to label human MSCs with 111In for scintigraphic tracking of stem cells delivered to the heart in clinical trials without affecting the viability and differentiation capacity of the MSCs. This creates an important tool for the control of stem cell delivery and dose response in clinical cardiovascular trials.

Keywords

Mesenchymal stem cells Indium-111 Imaging Cell cultures Endothelial differentiation