Labelling of human mesenchymal stem cells with indium-111 for SPECT imaging: effect on cell proliferation and differentiation
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Stem cell therapy seems to be a new treatment option within cardiac diseases to improve myocardial perfusion and function. However, the delivery and traceability of the cells represent a problem. Radioactive labelling with 111In could be a method for tracking mesenchymal stem cells (MSCs). However, 111In could influence the viability and differentiation capacity of MSCs, which would limit its use. Therefore, the aim of this study was to evaluate the influence of 111In labelling in doses relevant for SPECT imaging in humans on the viability and differentiation capacity of human MSCs.
Methods and results
Human MSCs isolated from bone marrow were incubated with 111In-tropolone (15–800 Bq/cell). The labelling efficiency was approximately 25% with 30 Bq/cell 111In. The MSC doubling time was 1.04±0.1 days and was not influenced by 111In within the range 15–260 Bq/cell. Using 30 Bq 111In/cell it was possible to label MSCs to a level relevant for clinical scintigraphic use. With this dose, 111In had no effect on characteristic surface and intracellular markers of cultured MSCs analysed both by flow cytometry and by real-time polymerase chain reaction. Further, the labelled MSCs differentiated towards endothelial cells and formed vascular structures.
It is possible to label human MSCs with 111In for scintigraphic tracking of stem cells delivered to the heart in clinical trials without affecting the viability and differentiation capacity of the MSCs. This creates an important tool for the control of stem cell delivery and dose response in clinical cardiovascular trials.
- Labelling of human mesenchymal stem cells with indium-111 for SPECT imaging: effect on cell proliferation and differentiation
European Journal of Nuclear Medicine and Molecular Imaging
Volume 33, Issue 10 , pp 1171-1177
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Mesenchymal stem cells
- Cell cultures
- Endothelial differentiation
- Industry Sectors
- Author Affiliations
- 1. Stem Cell Laboratory 9312, The Heart Centre, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
- 2. Department of Clinical Physiology, Nuclear Medicine & PET, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark
- 4. CVU Oeresund, Faculty of Medical Laboratory Sciences,, Copenhagen, Denmark
- 3. Cluster for Molecular Imaging, University of Copenhagen, Copenhagen, Denmark
- 5. Cardiac Catheterisation Laboratory 2014,The Heart Centre, Rigshospitalet, Blegdamsvej 9, 2100, Copenhagen, Denmark