Effective local control of prostate cancer by intratumoral injection of 166Ho-chitosan complex (DW-166HC) in rats

  • Cheol Kwak
  • Sung Kyu Hong
  • Seung Kyoo Seong
  • Jei Man Ryu
  • Moon Soo Park
  • Sang Eun Lee
Original Article

DOI: 10.1007/s00259-005-1892-y

Cite this article as:
Kwak, C., Hong, S.K., Seong, S.K. et al. Eur J Nucl Med Mol Imaging (2005) 32: 1400. doi:10.1007/s00259-005-1892-y

Abstract

Purpose

The aim of this study was to evaluate the therapeutic effect and morphological alterations resulting from 166Ho-chitosan complex (DW-166HC) in an animal model of prostate cancer.

Methods

First, in a subcutaneous tumor model, 80 rats were randomly divided into four groups (n=20 in each group), and intratumoral injections of 0.05 ml (normal saline in group 1, 165Ho-chitosan complex solution in group 2, DW-166HC solution (10 mCi) in group 3, and DW-166HC solution (20 mCi) in group 4) were performed when the tumor measured approximately 1 cm along its long axis in each group. Further, in an orthotopic tumor model, 40 rats were similarly randomly divided into four groups (n=10 in each group), and intraprostatic injections of 0.05 ml [PBS in group 1, 165Ho-chitosan complex solution in group 2, DW-166HC solution (0.5 mCi) in group 3 and DW-166HC solution (1 mCi) in group 4] were performed at 1 week after implantation of the AIT cell line in the ventral prostate.

Results

In the subcutaneous tumor model, mean tumor weights of groups 3 and 4 were significantly lower than those of groups 1 and 2 at 2 and 4 weeks post injection (p<0.05). At 2 and 4 weeks after injection in the orthotopic tumor model, the mean weights of the prostate, including tumor, in groups 3 and 4 were also significantly lower than those in groups 1 and 2 (p<0.05). No adverse injury was seen in adjacent organs at histopathologic examination.

Conclusion

Intratumoral injection of the beta-emitting radionuclide 166Ho as a form of complex solution with chitosan appears to be a promising alternative therapeutic modality for the local control of prostate cancer.

Keywords

Prostate cancerRadionuclidesBeta emittersHolmiumChitosan

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Cheol Kwak
    • 1
  • Sung Kyu Hong
    • 1
  • Seung Kyoo Seong
    • 2
  • Jei Man Ryu
    • 2
  • Moon Soo Park
    • 3
  • Sang Eun Lee
    • 4
  1. 1.Department of UrologySeoul National University College of Medicine and Clinical Research Institute, Seoul National University HospitalSeoulKorea
  2. 2.Dong Wha Pharmaceutical Industrial Company LimitedAnyangKorea
  3. 3.Seun Leung Top Urology ClinicSeoulKorea
  4. 4.Department of UrologySeoul National University College of MedicineSeoulKorea