Voxel-based statistical analysis of cerebral glucose metabolism in the rat cortical deafness model by 3D reconstruction of brain from autoradiographic images
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Animal models of cortical deafness are essential for investigation of the cerebral glucose metabolism in congenital or prelingual deafness. Autoradiographic imaging is mainly used to assess the cerebral glucose metabolism in rodents. In this study, procedures for the 3D voxel-based statistical analysis of autoradiographic data were established to enable investigations of the within-modal and cross-modal plasticity through entire areas of the brain of sensory-deprived animals without lumping together heterogeneous subregions within each brain structure into a large region of interest.
Thirteen 2-[1-14C]-deoxy-D-glucose autoradiographic images were acquired from six deaf and seven age-matched normal rats (age 6–10 weeks). The deafness was induced by surgical ablation. For the 3D voxel-based statistical analysis, brain slices were extracted semiautomatically from the autoradiographic images, which contained the coronal sections of the brain, and were stacked into 3D volume data. Using principal axes matching and mutual information maximization algorithms, the adjacent coronal sections were co-registered using a rigid body transformation, and all sections were realigned to the first section. A study-specific template was composed and the realigned images were spatially normalized onto the template. Following count normalization, voxel-wise t tests were performed to reveal the areas with significant differences in cerebral glucose metabolism between the deaf and the control rats.
Continuous and clear edges were detected in each image after registration between the coronal sections, and the internal and external landmarks extracted from the spatially normalized images were well matched, demonstrating the reliability of the spatial processing procedures. Voxel-wise t tests showed that the glucose metabolism in the bilateral auditory cortices of the deaf rats was significantly (P<0.001) lower than that in the controls. There was no significantly reduced metabolism in any other area, and no area showed a significant increase in metabolism in the deaf rats with the same threshold, demonstrating the high localization accuracy and specificity of the method developed in this study.
This study established new procedures for the 3D reconstruction and voxel-based analysis of autoradiographic data which will be useful for examining the cerebral glucose metabolism in a rat cortical deafness model.
- Ito J, Sakakibara J, Iwasaki Y, Yonekura Y. Positron emission tomography of auditory sensation in deaf patients and patients with cochlear implants. Ann Otol Rhinol Laryngol 1993;102:797–801.
- Catalán-Ahumada M, Deggouj N, De Volder A, Melin J, Michel C, Veraart C. High metabolic activity demonstrated by positron emission tomography in human auditory cortex in case of deafness of early onset. Brain Res 1993;623:287–92.
- Deggouj N, Devolder A, Catalan M, Melin J, Michel C, Gersdorff M, et al. Positron emission tomography in deaf patients at rest. Adv Otorhinolaryngol 1995;50:31–7.
- Lee DS, Lee JS, Oh SH, Kim SK, Kim JW, Chung JK, et al. Cross-modal plasticity and cochlear implants. Nature 2001;409:149–50.
- Lee JS, Lee DS, Oh SH, Kim CS, Kim JW, Hwang CH, et al. PET evidence of neuroplasticity in adult auditory cortex of postlingual deafness. J Nucl Med 2003;44:1435–9.
- Bavelier D, Neville HJ. Cross-modal plasticity: where and how? Nature Rev Neurosci 2002;3:443–52.
- Cordes M, Wszolek ZK. Deafness and cerebral plasticity. J Nucl Med 2003;44:1440–2.
- Ishizu K, Mukai T, Yonekura Y, Pagani M, Fujita T, Magata Y, et al. Ultra-high resolution SPECT system using four pinhole collimators for small animal studies. J Nucl Med 1995;36:2282–7.
- Weber DA, Ivanovich M. Pinhole SPECT: ultra-high resolution imaging for small animal studies. J Nucl Med 1995;36:2287–9.
- Chatziioannou AF. Molecular imaging of small animals with dedicated PET tomographs. Eur J Nucl Med Mol Imaging 2002;29:98–114. CrossRef
- Lewis JS, Achilefu S, Garbow JR, Laforest R, Welch MJ. Small animal imaging. Current technology and perspectives for oncological imaging. Eur J Cancer 2002;38:2173–88. CrossRef
- Friston KJ, Holmes AP, Worsley KJ, Poline J-P, Frith CD, Frackowiak RSJ. Statistical parametric maps in functional imaging: a general linear approach. Hum Brain Mapp 1995;2:189–210.
- Nguyen PT, Holschneider DP, Maarek JM, Yang J, Mandelkern MA. Statistical parametric mapping applied to an autoradiographic study of cerebral activation during treadmill walking in rats. Neuroimage 2004;23:252–9.
- Ahn SH, Oh SH, Lee JS, Jeong JM, Lim D, Lee DS, et al. Changes of 2-deoxyglucose uptake in the rat auditory pathway after bilateral ablation of the cochlea. Hear Res 2004;196:33–8.
- Sharma A, Dorman MF, Spahr AJ. A sensitive period for the development of the central auditory system in children with cochlear implants: implications for age of implantation. Ear Hear 2002;23:532–9.
- Alpert NM, Bradshaw JF, Kennedy D, Correia JA. The principal axes transformation—a method for image registration. J Nucl Med 1990;31:1717–22.
- Maes F, Collignon A, Vandermeulen D, Marchal G, Suetens P. Multimodality image registration by maximization of mutual information. IEEE Trans Med Imaging 1997;16:187–98. CrossRef
- Gonzalez RC, Woods RE. Digital image processing. Reading, MA: Addison-Wesley; 1992. p. 458–61.
- Kim B, Boes JL, Frey KA, Meyer CR. Mutual information for automated unwarping of rat brain autoradiographs. Neuroimage 1997;5:31–40.
- Friston KJ, Ashburner J, Frith CD, Poline J-B, Heather JD, Frackowiak RSJ. Spatial registration and normalization of images. Hum Brain Mapp 1995;2:165–89.
- Ashburner J, Friston KJ. Nonlinear spatial normalization using basis functions. Hum Brain Mapp 1999;7:254–66. CrossRef
- Toga AW, Thompson PM. Maps of the brain. Anat Rec 2001;265:37–53.
- Karas GB, Burton EJ, Rombouts SA, van Schijndel RA, O’Brien JT, Scheltens P, et al. A comprehensive study of gray matter loss in patients with Alzheimer’s disease using optimized voxel-based morphometry. Neuroimage 2003;18:895–907.
- Paxinos G, Watson C. The rat brain in stereotaxic coordinates. Orlando, FL: Academic Press; 1986:x-xi.
- Canny J. A computational approach to edge detection. IEEE Trans Pattern Anal Machine Intell 1986;8:679–98.
- Hibbard LS, Hawkins RA. Objective image alignment for three-dimensional reconstruction of digital autoradiograms. J Neurosci Methods 1988;26:55–74.
- Zhao W, Young TY, Ginsberg MD. Registration and three-dimensional reconstruction of autoradiographic images by the disparity analysis method. IEEE Trans Med Imaging 1993;12:782–91.
- Goldszal AF, Tretiak OJ, Hand PJ, Bhasin S, McEachron DL. Three-dimensional reconstruction of activated columns from 2-[14C]deoxy-D-glucose data. Neuroimage 1995;2:9–20.
- Nikou C, Heitz F, Nehlig A, Namer IJ, Armspach JP. A robust statistics-based global energy function for the alignment of serially acquired autoradiographic sections. J Neurosci Methods 2003;124:93–102.
- Zhao W, Ginsberg MD, Smith DW. Three-dimensional quantitative autoradiography by disparity analysis: theory and application to image averaging of local cerebral glucose utilization. J Cereb Blood Flow Metab 1995;15:552–65.
- Ginsberg MD, Zhao W, Singer JT, Alonso OF, Loor-Estades Y, Dietrich WD, et al. Computer-assisted image-averaging strategies for the topographic analysis of in situ hybridization autoradiographs. J Neurosci Methods 1996;68:225–33.
- Rubins DJ, Melega WP, Lacan G, Way B, Plenevaux A, Luxen A, et al. Development and evaluation of an automated atlas-based image analysis method for microPET studies of the rat brain. Neuroimage 2003;20:2100–18.
- Voxel-based statistical analysis of cerebral glucose metabolism in the rat cortical deafness model by 3D reconstruction of brain from autoradiographic images
European Journal of Nuclear Medicine and Molecular Imaging
Volume 32, Issue 6 , pp 696-701
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Cerebral glucose metabolism
- Voxel-based analysis
- Industry Sectors
- Author Affiliations
- 1. Department of Nuclear Medicine, Seoul National University College of Medicine, 28 Yungun-Dong, Chongno-Ku, Seoul, 110-799, Korea
- 2. Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul, Korea
- 3. Department of Otolaryngology, Head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea