Applied Microbiology and Biotechnology

, Volume 57, Issue 1, pp 216–219

Production of a heterologous proteinase A by Saccharomyces kluyveri

  •  K. Møller
  •  L. Tidemand
  •  J. Winther
  •  L. Olsson
  •  J. Piškur
  •  J. Nielsen
Original Paper

DOI: 10.1007/s002530100680

Cite this article as:
Møller, K., Tidemand, L., Winther, J. et al. Appl Microbiol Biotechnol (2001) 57: 216. doi:10.1007/s002530100680

Abstract.

In order to evaluate the potential of Saccharomyces kluyveri for heterologous protein production, S. kluyveri Y159 was transformed with a S. cerevisiae-based multi-copy plasmid containing the S. cerevisiaePEP4 gene, which encodes proteinase A, under the control of its native promoter. As a reference, S. cerevisiae CEN.PK 113-5D was transformed with the same plasmid and the two strains were characterised in batch cultivations on glucose. The glucose metabolism was found to be less fermentative in S. kluyveri than in S. cerevisiae. The yield of ethanol on glucose was 0.11 g/g in S. kluyveri, compared to a yield of 0.40 g/g in S. cerevisiae. Overexpression of PEP4 led to the secretion of active proteinase A in both S. kluyveri and S. cerevisiae. The yield of active proteinase A during growth on glucose was found to be 3.6-fold higher in S. kluyveri than in the S. cerevisiae reference strain.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  K. Møller
    • 1
  •  L. Tidemand
    • 1
  •  J. Winther
    • 2
  •  L. Olsson
    • 1
  •  J. Piškur
    • 3
  •  J. Nielsen
    • 1
  1. 1.Center for Process Biotechnology, BioCentrum–DTU, Technical University of Denmark, 2800 Lyngby, Denmark
  2. 2.Carlsberg Laboratory, Gamle Carlsberg Vej 10, 2500 Copenhagen Valby, Denmark
  3. 3.Section for Molecular Microbiology, BioCentrum–DTU, Technical University of Denmark, 2800 Lyngby, Denmark

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