Applied Microbiology and Biotechnology

, Volume 56, Issue 3, pp 545–549

Tetrachloroethylene, trichloroethylene, and chlorinated phenols induce toluene-o-xylene monooxygenase activity in Pseudomonas stutzeri OX1

  •  D. Ryoo
  •  H. Shim
  •  F. Arenghi
  •  P. Barbieri
  •  T. Wood
Original Paper

DOI: 10.1007/s002530100675

Cite this article as:
Ryoo, D., Shim, H., Arenghi, F. et al. Appl Microbiol Biotechnol (2001) 56: 545. doi:10.1007/s002530100675

Abstract.

Pseudomonas stutzeri OX1 naphthalene-oxidation activity is induced 3.0-fold by tetrachloroethylene (PCE) and 3.1-fold by trichloroethylene (TCE) at 100 µM. With the mutant P. stutzeri M1, which does not express toluene-o-xylene monooxygenase (ToMO, product of the tou operon), no naphthalene-oxidation activity induction by PCE and TCE was found; hence, PCE and TCE induce ToMO of P. stutzeri OX1. The chlorinated phenols 2-, 3-, and 4-chlorophenol induced ToMO expression 0.58-, 0.23- and 0.37-fold, respectively, compared to the direct inducer of the pathway, o-cresol. Using P. putida PaW340 (pPP4062, pFP3028), which has the tou promoter fused to the reporter catechol-2,3-dioxygenase, and the regulator gene touR, it was determined that the tou promoter was induced directly 5.7-, 7.1-, and 5.1-fold for 2-, 3-, and 4-chlorophenol, respectively (compared to an 8.8-fold induction with o-cresol). In addition, it was found that TCE and PCE do not directly induce the tou pathway and that components other than the tou structural and regulatory genes are necessary for induction. Gas chromatography results also showed that 100 µM TCE induced its own degradation (8–9%) in 16 h in P. stutzeri OX1, and all of the stoichiometric chloride from the degraded TCE was detected in solution.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  D. Ryoo
    • 1
  •  H. Shim
    • 1
  •  F. Arenghi
    • 2
  •  P. Barbieri
    • 2
  •  T. Wood
    • 1
  1. 1.Department of Chemical Engineering, University of Connecticut, Storrs, CT 06269–3222, USA
  2. 2.Dipartimento di Genetica e di Biologia dei Microrganismi Universita' degli studi di Milano, 20133 Milan, Italy
  3. 3.Present address: Department of Environmental Engineering, Jeonju University, Jeonju 560–759, Korea
  4. 4.Present address: Dipartimento di Biologia Strutturale e Funzionale, Università dell'Insubria, 21100 Varese, Italy
  5. 5.Present address: Department of Civil and Environmental Engineering, Hanyang University, Kyungkido 425–791, Korea