Applied Microbiology and Biotechnology

, Volume 56, Issue 1, pp 225–232

Enhanced formation of laccase activity by the white-rot fungus Trametes pubescens in the presence of copper

  •  C. Galhaup
  •  D. Haltrich
Original Paper

DOI: 10.1007/s002530100636

Cite this article as:
Galhaup, C. & Haltrich, D. Appl Microbiol Biotechnol (2001) 56: 225. doi:10.1007/s002530100636

Abstract.

The white-rot fungus Trametes pubescens MB 89 has been identified as an outstanding, although not-yet-described, producer of the industrially important enzyme laccase. Extracellular laccase formation could be greatly stimulated by the addition of Cu(II) to a simple, glucose-based culture medium. Using optimum Cu concentrations (1.5–2.0 mM), maximum values for laccase activity of approximately 65 U/ml were obtained. The synthesis of the laccase protein depended on the presence of Cu in the medium as shown by Western blot analysis. Copper had to be supplemented during the exponential phase of growth for its maximal effect; addition during the stationary phase, during which laccase activity is predominantly formed, resulted in markedly reduced laccase productivity. As was shown by X-ray microanalysis of T. pubescens mycelia obtained from a laboratory fermentation, Cu was rapidly taken up by the fungal biomass. A possible explanation for this stimulatory effect of Cu on laccase biosynthesis could be a role for this enzyme activity in melanin synthesis. The stimulatory effect of Cu on laccase synthesis was also effective for several other basidiomycetes and hence could be used as a simple method to boost the production of this enzyme.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  C. Galhaup
    • 1
  •  D. Haltrich
    • 1
  1. 1.Division of Biochemical Engineering, Institute of Food Technology, University of Agricultural Sciences Vienna (Universität für Bodenkultur Wien), Muthgasse 18, 1190 Vienna, Austria