Applied Microbiology and Biotechnology

, Volume 55, Issue 6, pp 680–685

Efficient pyruvate production by a multi-vitamin auxotroph of Torulopsis glabrata: key role and optimization of vitamin levels

  •  Y. Li
  •  J. Chen
  •  S.-Y. Lun
  •  X.-S. Rui
Original Paper

DOI: 10.1007/s002530100598

Cite this article as:
Li, Y., Chen, J., Lun, SY. et al. Appl Microbiol Biotechnol (2001) 55: 680. doi:10.1007/s002530100598
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Abstract.

A multi-vitamin auxotroph, Torulopsis glabrata strain WSH-IP303, which can use ammonium chloride as a sole nitrogen source for pyruvate production, was selected. To optimize pyruvate yield and productivity, a simple but useful, orthogonal design method, was used to investigate the relationship between thiamine, nicotinic acid, pyridoxine, biotin, and riboflavin. Thiamine was confirmed to be the most important factor affecting pyruvate production. When the concentration of thiamine was 0.01 mg/l or 0.015 mg/l, glucose consumption was improved by increasing the nicotinic acid concentration. When the concentrations of nicotinic acid, thiamine, pyridoxine, biotin, and riboflavin were 8.0, 0.015, 0.4, 0.04, and 0.1 mg/l, respectively, pyruvate concentration and yield reached 52 g/l and 0.52 g/g, respectively, in a 48-h flask culture. By employing a combination of the optimum vitamin concentrations, a batch culture was conducted in a 2.5-l fermentor with an initial glucose concentration of 112 g/l; and the pyruvate concentration reached 69 g/l after 56 h (yielding 0.62 g/g).

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  •  Y. Li
    • 1
  •  J. Chen
    • 1
  •  S.-Y. Lun
    • 1
  •  X.-S. Rui
    • 1
  1. 1.Laboratory of Environmental Biotechnology, School of Biotechnology, Wuxi University of Light Industry, Wuxi 214036, P.R. China
  2. 2.Present address: Changzhou Shuguang Chemical Engineering Co. Ltd, Changzhou 213016, P.R. China

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