Applied Microbiology and Biotechnology

, Volume 53, Issue 1, pp 30–35

Enhanced sterol-acyl transferase activity promotes sterol accumulation in Saccharomyces cerevisiae

  • T. Polakowski
  • R. Bastl
  • U. Stahl
  • C. Lang
ORIGINAL PAPER

DOI: 10.1007/s002530051610

Cite this article as:
Polakowski, T., Bastl, R., Stahl, U. et al. Appl Microbiol Biotechnol (1999) 53: 30. doi:10.1007/s002530051610

Abstract

The sterol-acyl transferase encoded by the gene ARE2 was transcriptionally deregulated in the yeast Saccharomyces cerevisiae to understand its role in sterol storage and sterol enrichment. Our results show that sterols can indeed be enriched in yeast by enhancing the capacity of the cells to esterify sterols. ARE2 overexpression had no impact on the accumulation of the early sterols such as lanosterol, but influenced the later intermediates and the end product ergosterol. Thus an enhanced conversion of free sterols to their esterified counterparts may provide a tool to increase the overall sterol content of the yeast cell. We have previously shown that the overexpression of a truncated version of the key enzyme of the early sterol pathway, HMG-CoA reductase (HMG1), leads to an increase in the early sterols such as lanosterol and zymosterol. The simultaneous deregulation of both genes in one strain produces a cumulative effect in that both early and late sterols are enhanced. Karmellae-like structures can be detected when Are2p is overexpressed. Are2p therefore constitutes a new member of the karmellae-inducing protein family.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • T. Polakowski
    • 1
  • R. Bastl
    • 2
  • U. Stahl
    • 1
  • C. Lang
    • 1
  1. 1.Institut für Biotechnologie, FG Mikrobiologie und Genetik, Technische Universität Berlin, Gustav-Meyer-Allee 25, D-13355 Berlin, Germany e-mail: C.Lang@LB.TU-Berlin.de Tel.: +49-30-31472751 Fax: +49-30-31472922DE
  2. 2.Institut für Lebensmittelchemie, Technische Universität Berlin, Gustav-Meyer-Allee 25, D-13355 Berlin, GermanyDE