MINI-REVIEW

Applied Microbiology and Biotechnology

, Volume 51, Issue 4, pp 422-430

High-cell-density cultivation of microorganisms

  • D. RiesenbergAffiliated withHans Knöll Institute for Natural Product Research, Beutenbergstraße 11, 07745 Jena, Germany e-mail: driesenb@pmail.hki-jena.de Tel.: +49 3641 65 6811 Fax: +49 3641 65 6800
  • , R. GuthkeAffiliated withHans Knöll Institute for Natural Product Research, Beutenbergstraße 11, 07745 Jena, Germany e-mail: driesenb@pmail.hki-jena.de Tel.: +49 3641 65 6811 Fax: +49 3641 65 6800

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

High-cell-density cultivation (HCDC) is required to improve microbial biomass and product formation substantially. An overview of HCDC is given for microorganisms including bacteria, archae and eukarya (yeasts). Problems encountered by HCDC and their possible solutions are discussed. Improvements of strains, different types of bioreactors and cultivation strategies for successful HCDC are described. Stirred-tank reactors with and without cell retention, a dialysis-membrane reactor, a gas-lift reactor and a membrane cyclone reactor used for HCDC are outlined. Recently modified traditional feeding strategies and new ones are included, in particular those for unlimited growth to very dense cultures. Emphasis is placed on robust fermentation control because of the growing industrial interest in this field. Therefore, developments in the application of multivariate statistical control, artificial neural networks, fuzzy control and knowledge-based supervision (expert systems) are summarized. Recent advances using Escherichia coli– the pioneer organism for HCDC – are outlined.