Applied Microbiology and Biotechnology

, Volume 51, Issue 2, pp 176–184

N -glycosylation is involved in the sensitivity of Saccharomyces cerevisiae to HM-1 killer toxin secreted from Hansenula mrakii IFO 0895

  • T. Kimura
  • T. Komiyama
  • Y. Furuichi
  • Y. Iimura
  • S. Karita
  • K. Sakka
  • K. Ohmiya
ORIGINAL PAPER

DOI: 10.1007/s002530051379

Cite this article as:
Kimura, T., Komiyama, T., Furuichi, Y. et al. Appl Microbiol Biotechnol (1999) 51: 176. doi:10.1007/s002530051379

Abstract

Saccharomyces cerevisiae rhk mutants were previously shown to have a phenotype that is resistant to HM-1 killer toxin secreted from Hansenula mrakii IFO 0895. The RHK1/ALG3 gene encodes a mannosyltransferase that is involved in the synthesis of an oligosaccharide in protein N-glycosylation. Previously, this gene was cloned and shown to complement the rhk1 mutation. In this study, the RHK2 gene, which complements the rhk2 mutation, was cloned. The RHK2 gene was found to be identical to the essential gene STT3, which encodes a subunit of the oligosaccharyltransferase complex. This complex transfers the core oligosaccharide to proteins. The rhk2 mutants showed supersensitivity to several drugs (Calcofluor White, caffeine and FK506), suggesting that these strains have cell-wall defects. Activity staining of invertase in an acrylamide gel indicated that it was underglycosylated. These results suggest that one or more mannoproteins are involved in the cytocidal process of HM-1.

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • T. Kimura
    • 1
  • T. Komiyama
    • 3
  • Y. Furuichi
    • 4
  • Y. Iimura
    • 5
  • S. Karita
    • 2
  • K. Sakka
    • 1
  • K. Ohmiya
    • 1
  1. 1.Faculty of Bioresources, Mie University, Tsu, Mie 514-8507, Japan e-mail: t-kimura@bio.mie-u.ac.jp Tel.: +81-59-231-9606 Fax: +81-59-231-9634JP
  2. 2.Center for Molecular Biology and Genetics, Mie University, Tsu, Mie 514-8507, JapanJP
  3. 3.Department of Biochemistry, Niigata College of Pharmacy, Niigata 950-21, JapanJP
  4. 4.AGENE Research Institute, Kajiwara, Kamakura, Kanagawa 214, JapanJP
  5. 5.Faculty of Engineering, Yamanashi University, Kofu, Yamanashi 400-0016, JapanJP