Applied Microbiology and Biotechnology

, Volume 49, Issue 4, pp 450–454

Hydrogen production with high yield and high evolution rate by self-flocculated cells of Enterobacter aerogenes in a packed-bed reactor

  • M. A. Rachman
  • Y. Nakashimada
  • T. Kakizono
  • N. Nishio
SHORT CONTRIBUTION

DOI: 10.1007/s002530051197

Cite this article as:
Rachman, M., Nakashimada, Y., Kakizono, T. et al. Appl Microbiol Biotechnol (1998) 49: 450. doi:10.1007/s002530051197

Abstract

Continuous hydrogen gas evolution by self-flocculated cells of Enterobacter aerogenes, a natural isolate HU-101 and its mutant AY-2, was performed in a packed-bed reactor under glucose-limiting conditions in a minimal medium. The flocs that formed during the continuous culture were retained even when the dilution rate was increased to 0.9 h−1. The H2 production rate increased linearly with increases in the dilution rate up to 0.67 h−1, giving maximum H2 production rates of 31 and 58 mmol l−1 h−1 in HU-101 and AY-2 respectively, at a dilution rate of more than 0.67 h−1. The molar H2 yield from glucose in AY-2 was maintained at about 1.1 at dilution rates between 0.08 h−1 and 0.67 h−1, but it decreased rapidly at dilution rates more than 0.8 h−1.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • M. A. Rachman
    • 1
  • Y. Nakashimada
    • 1
  • T. Kakizono
    • 1
  • N. Nishio
    • 1
  1. 1.Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University, Kagamiyama 1-4-1, Higashi Hiroshima 739, Japan Fax: +81 824-22-7162JP

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