Applied Microbiology and Biotechnology

, Volume 49, Issue 1, pp 117–121

Utilization of sorbed compounds by microorganisms specifically isolated for that purpose

  • W.-C. Tang
  • J. C. White
  • M. Alexander
ORIGINAL PAPER

DOI: 10.1007/s002530051147

Cite this article as:
Tang, W., White, J. & Alexander, M. Appl Microbiol Biotechnol (1998) 49: 117. doi:10.1007/s002530051147

Abstract

A bacterium obtained by enrichment on nonsorbed phenanthrene was unable to degrade phenanthrene sorbed to polyacrylic beads and had little activity on phenanthrene sorbed to lake-bottom sediment. A bacterium obtained by enrichment on phenanthrene sorbed to polyacrylic beads readily mineralized the compound sorbed to the beads or the sediment. Degradation by the second bacterium of phenanthrene sorbed to beads 38–63 μm or 63–150 μm in diameter was more rapid than the rate of desorption of the hydrocarbon in the absence of the bacterium. Little degradation of sorbed, nonleachable phenanthrene in soil was effected by another isolate obtained by enrichment with the nonsorbed hydrocarbon, but a mixed culture and the bacterium obtained by enrichment on the sorbed compound extensively degraded phenanthrene. Because microorganisms specifically obtained for their capacity to degrade sorbed phenanthrene are more active than species not specialized for use of the bound compound, we suggest that microorganisms enriched on nonsorbed compounds may not be appropriate for evaluation of biodegradation and bioremediation of sorbed compounds.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • W.-C. Tang
    • 1
  • J. C. White
    • 1
  • M. Alexander
    • 1
  1. 1.Laboratory of Soil Microbiology, Department of Soil, Crop and Atmospheric Sciences, 708 Bradfield Hall, Cornell University, Ithaca, NY 14853, USA Tel.: +1 607 255 1717 Fax: +1 607 255 2644US