Applied Microbiology and Biotechnology

, Volume 49, Issue 1, pp 66–71

Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast

  • T. Polakowski
  • U. Stahl
  • C. Lang
ORIGINAL PAPER

DOI: 10.1007/s002530051138

Cite this article as:
Polakowski, T., Stahl, U. & Lang, C. Appl Microbiol Biotechnol (1998) 49: 66. doi:10.1007/s002530051138

Abstract

The enzyme 3-hydroxy-3-methylglutaryl-coenzyme-A (HMG-CoA) reductase is known as the rate-limiting enzyme in early sterol biosynthesis in eukaryotic cells. To eliminate this regulation in the yeast Saccharomyces cerevisiae, a truncated HMG1 gene, producing a form of the enzyme that lacks the membrane-binding region (i.e. amino acids 1–552), was constructed and overexpressed in this yeast. The transformed strains accumulated large amounts of the sterol precursor squalene, while the levels of ergosterol and a number of other sterol compounds were only slightly elevated. These findings suggest that HMG-CoA reductase is not the only rate-limiting step in sterol synthesis and its overexpression cannot significantly influence this pathway beyond the sterol precursor squalene.

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • T. Polakowski
    • 1
  • U. Stahl
    • 1
  • C. Lang
    • 1
  1. 1.Technische Universität Berlin, Institut für Biotechnologie, Fachgebiet Mikrobiologie und Genetik, Gustav-Meyer-Allee 25, 13355 Berlin, Germany Tel.: +49 30 31472751 Fax: +49 30 31472922 e-mail: C.Lang@LB.TU-Berlin.deDE