, Volume 49, Issue 1, pp 31-38

Purification and characterization of recombinant spider silk expressed in Escherichia coli

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


A partial cDNA clone, from the 3′ end of the dragline silk gene was isolated from Nephila clavipes major ampullate glands. This clone contains a 1.7-kb insert, consisting of a repetitive coding region of 1.4-kb and a 0.3-kb nonrepetitive coding region; 1.5-kb of the 1.7-kb fragment was cloned into Escherichia coli and a␣43-kDa recombinant silk protein was expressed. Characterization of the purified protein by Western blot, amino acid composition analysis, and matrix-assisted laser desorption ionization/time-of-flight mass spectrometry confirms it to be spider dragline silk.

Received: 7 April 1997 / Received revision: 24 July 1997 / Accepted: 25 August 1997