Applied Microbiology and Biotechnology

, Volume 47, Issue 5, pp 610–614

Multi-substrate growth kinetics of Pseudomonas putida for phenol removal

  • Ş. Şeker
  • H. Beyenal
  • B. Salih
  • A. Tanyolaç
ORIGINAL PAPER

DOI: 10.1007/s002530050982

Cite this article as:
Şeker, Ş., Beyenal, H., Salih, B. et al. Appl Microbiol Biotechnol (1997) 47: 610. doi:10.1007/s002530050982

Abstract

The biodegradation of phenol by a pure culture of Pseudomonas putida was investigated in a continuously fed stirred-tank reactor, under aerobic conditions. The dilution rate was varied between 0.0174 h−1 and 0.278 h−1, covering a wide range of dissolved oxygen and the inhibition region of phenol. Through non-linear analysis of the data, a dual-substrate growth kinetics, Haldane kinetics for phenol and Monod kinetics for oxygen, was derived with high correlation coefficients. Respective biokinetic parameters were evaluated as μm = 0.569 h−1, Kp = 18.539 mg/l, Ki = 99.374 mg/l, Ko = 0.048 mg/l, Yx/p = 0.521 g microorganism/g phenol and Yx/o = 0.338 g microorganism/g oxygen, being in good agreement with other studies in the literature. Maintenance factors for both phenol and oxygen were calculated for the first time for P. putida while the saturation coefficient for oxygen, Ko, was genuinely evaluated from the constructed model, not imported or adapted from other studies as reported in the literature. All pertinent biokinetic parameters for P. putida have been calculated from continuous system data, which are most appropriate for use in continuous bioprocess applications.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Ş. Şeker
    • 1
  • H. Beyenal
    • 1
  • B. Salih
    • 2
  • A. Tanyolaç
    • 1
  1. 1.Hacettepe University, Chemical Engineering Department, Beytepe, 06532 Ankara, Turkey. Fax: (312) 2352330 e-mail: tanyolac@eti.cc.hun.edu.trTR
  2. 2.Hacettepe University, Chemistry Department, Beytepe, 06532 Ankara, Turkey.TR