Applied Microbiology and Biotechnology

, Volume 47, Issue 1, pp 33–39

Production of synthetic spider dragline silk protein in Pichia pastoris

  • S. R. Fahnestock
  • L. A. Bedzyk
ORIGINAL PAPER

DOI: 10.1007/s002530050884

Cite this article as:
Fahnestock, S. & Bedzyk, L. Appl Microbiol Biotechnol (1997) 47: 33. doi:10.1007/s002530050884

Abstract

The methylotrophic yeast Pichia pastoris was tested as a host for the production of long, repetitive protein polymers. Synthetic genes for a designed analog of a spider dragline silk protein were readily expressed at high levels under control of the methanol-inducible AOX1 promoter. Transformants containing multiple gene copies produced elevated levels of silk protein, but of a variety of altered sizes as a result of gene rearrangements at the time of transformation. Genes up to 3000 codons in length or longer could be expressed with no evidence of the prevalent truncated synthesis observed for similar genes in Escherichia coli, though genes longer than 1600 codons were expressed less efficiently than shorter genes. Silk-producing P. pastoris strains were stable without selection for at least 100 doublings.

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • S. R. Fahnestock
    • 1
  • L. A. Bedzyk
    • 1
  1. 1.Central Science and Engineering Laboratories, E.I. DuPont de Nemours & Co., Experimental Station, Wilmington, DE 19880–0328, USA. Fax: +1 302 695 8412 e-mail: fahnessr@esvax.dnet.dupont.comUS