Applied Microbiology and Biotechnology

, Volume 45, Issue 6, pp 792–799

Manganese and malonate are individual regulators for the production of lignin and manganese peroxidase isozymes and in the degradation of lignin by Phlebia radiata

  • A. M. Moilanen
  • T. Lundell
  • T. Vares
  • A. Hatakka
ORIGINAL PAPER

DOI: 10.1007/s002530050764

Cite this article as:
Moilanen, A., Lundell, T., Vares, T. et al. Appl Microbiol Biotechnol (1996) 45: 792. doi:10.1007/s002530050764

Abstract

 The effects of high manganese [180 μM Mn(II)] concentration and addition of malonate (10 mM) were studied in nitrogen-limited cultures of the white-rot fungus, Phlebia radiata. High levels of manganese alone showed no systematic influence on the production of lignin peroxidase (LiP), manganese peroxidase (MnP) or laccase. In contrast, high-manganese containing cultures of P. radiata showed lower efficiency in the mineralization of 14C-ring-labelled synthetic lignin ([14C]DHP). The highest rates of mineralization, up to 30% in 18 days, were reached in low- manganese(2 μM)-containing cultures when malonate was omitted. Degradation of [14C]DHP was substantially restricted by the addition of malonate. The combination of high manganese and malonate resulted in increased levels of MnP and laccase production, whereas LiP production was repressed. Also, the profiles of expression of the MnP and LiP isozymes were affected. A new P. radiata MnP isozyme of pI 3.6 (MnP3) was found in the high-manganese cultures. Addition of malonate alone caused some repression but also stimulating effects on distinctive MnP and LiP isozymes. The results indicate that manganese and malonate are individual regulators of MnP and LiP expression and have different roles in the degradation of lignin by P. radiata.

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • A. M. Moilanen
    • 1
  • T. Lundell
    • 1
  • T. Vares
    • 1
  • A. Hatakka
    • 1
  1. 1.Department of Applied Chemistry and Microbiology, Division of Microbiology, PO Box 56, Biocenter 1, FIN-00014 University of Helsinki, Finland. Fax: +358 0 708 59322FI

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