Applied Microbiology and Biotechnology

, Volume 45, Issue 5, pp 700–709

Total reductive dechlorination of chlorobenzenes to benzene by a methanogenic mixed culture enriched from Saale river sediment

Authors

  • J. Nowak
    • Technische Universität Berlin, Institut für Technischen Umweltschutz, D-13355 Berlin, Germany
  • N. H. Kirsch
    • Institut für Lebensmittelchemie, Gustav-Meyer-Allee 25, D-13355 Berlin, Germany
  • W. Hegemann
    • Technische Universität Berlin, Institut für Technischen Umweltschutz, D-13355 Berlin, Germany
  • H.-J. Stan
    • Institut für Lebensmittelchemie, Gustav-Meyer-Allee 25, D-13355 Berlin, Germany
ORIGINAL PAPER

DOI: 10.1007/s002530050751

Cite this article as:
Nowak, J., Kirsch, N., Hegemann, W. et al. Appl Microbiol Biotechnol (1996) 45: 700. doi:10.1007/s002530050751

Abstract

 A methanogenic mixed culture enriched from Saale river sediment was able to transform all chlorobenzenes by reductive dechlorination via monochlorobenzene to unsubstituted benzene after a short lag phase of only 1 week. The formation of benzene was confirmed by GC/MS in pentane extracts of the sludge samples, as well as in the headspace gas of the batch cultures. The spectra of substrate utilization of the adapted cultures depended mainly on the substrates used for adaptation. Sediment microbes that had been adapted with a mixture of the three isomers of trichlorobenzene offered the widest spectrum for the utilization of chlorobenzenes in all chlorine stages. Reductive dechlorination was stimulated when the mixed cultures were supplemented with pyruvate and methanol.

Copyright information

© Springer-Verlag Berlin Heidelberg 1996