Applied Microbiology and Biotechnology

, Volume 53, Issue 3, pp 328–334

Degradation of the metal-cyano complex tetracyanonickelate (II) by Fusarium oxysporum N-10

  • H. Yanase
  • A. Sakamoto
  • K. Okamoto
  • K. Kita
  • Y. Sato
ORIGINAL PAPER

DOI: 10.1007/s002530050029

Cite this article as:
Yanase, H., Sakamoto, A., Okamoto, K. et al. Appl Microbiol Biotechnol (2000) 53: 328. doi:10.1007/s002530050029

Abstract

A fungus with the ability to utilize a metal-cyano compound, tetracyanonickelate (II) {K2[Ni (CN)4]; TCN}, as its sole source of nitrogen was isolated from soil and identified as Fusarium oxysporum N-10. Both intact mycelia and cell-free extract of the strain catalyzed hydrolysis of TCN to formate and ammonia and produced formamide as an intermediate, thereby indicating that a hydratase and an amidase sequentially participated in the degradation of TCN. The enzyme catalyzing the hydration of TCN was purified approximately ten-fold from the cell-free extract of strain N-10 with a yield of 29%. The molecular mass of the active enzyme was estimated to be 160 kDa. The enzyme appears to exist as a homotetramer, each subunit having a molecular mass of 40 kDa. The enzyme also catalyzed the hydration of KCN, with a cyanide-hydrating activity 2 × 104 times greater than for TCN. The kinetic parameters for TCN and KCN indicated that hydratase isolated from F. oxysporum was a cyanide hydratase able to utilize a broad range of cyano compounds and nitriles as substrates.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • H. Yanase
    • 1
  • A. Sakamoto
    • 1
  • K. Okamoto
    • 1
  • K. Kita
    • 1
  • Y. Sato
    • 2
  1. 1.Department of Biotechnology, Tottori University, Tottori 680-8552, Japan e-mail: yanase@bio.tottori-u.ac.jp Tel.: +81-857-315275 Fax: +81-857-310881JP
  2. 2.Frontier Technology Research Institute, Tokyo Gas, Yokohama 230-0045, JapanJP