Applied Microbiology and Biotechnology

, Volume 53, Issue 2, pp 219–223

Influence of a synbiotic mixture consisting of Lactobacillus acidophilus 74-2 and a fructooligosaccharide preparation on the microbial ecology sustained in a simulation of the human intestinal microbial ecosystem (SHIME reactor)

  • M. Gmeiner
  • W. Kneifel
  • K. D. Kulbe
  • R. Wouters
  • P. De Boever
  • L. Nollet
  • W. Verstraete
ORIGINAL PAPER

DOI: 10.1007/s002530050011

Cite this article as:
Gmeiner, M., Kneifel, W., Kulbe, K. et al. Appl Microbiol Biotechnol (2000) 53: 219. doi:10.1007/s002530050011

Abstract

Lactobacillus acidophilus 74-2, which is used in probiotic products, was administered, with fructooligosaccharide in a milk-based product, to the second vessel (duodenum/jejunum) of the SHIME reactor, an in vitro simulation of the human intestinal microbial ecology. The main focus of this study was to monitor the changes of the population density of selected bacterial species in the intestine and the changes of metabolic activities during the supplementation of L.acidophilus and fructooligosaccharide in the SHIME reactor. Interestingly, the addition of L. acidophilus 74-2 with fructooligosaccharide gave rise to an increase of bifidobacteria. Moreover, major positive changes occurred in the production of volatile fatty acids: a strong upward trend was observed especially in the case of butyric acid and propionic acid. Furthermore a noticeable increase of β-galactosidase activity was monitored, while the activity of β-glucuronidase, generally considered undesirable, declined.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. Gmeiner
    • 1
  • W. Kneifel
    • 1
  • K. D. Kulbe
    • 2
  • R. Wouters
    • 3
  • P. De Boever
    • 3
  • L. Nollet
    • 3
  • W. Verstraete
    • 3
  1. 1.Department of Dairy Research and Bacteriology, University of Agriculture, Gregor Mendel-Straße 33, A-1180 Vienna, AustriaAT
  2. 2.Institute of Food Technology, University of Agriculture, Muthgasse 18, A-1190 Vienna, AustriaAT
  3. 3.Laboratory of Microbial Ecology, Faculty of Agricultural and Applied Biological Science, University of Gent, Coupure Links 653, B-9000 Gent, Belgium e-mail: willy.verstraete@rug.ac.be Tel.: +32-9-264-59-76 Fax: +32-9-264-62-48BE