Applied Microbiology and Biotechnology

, Volume 53, Issue 2, pp 201–208

Production characteristics of interferon-α using an l-arabinose promoter system in a high-cell-density culture

  • H.-K. Lim
  • K.-H. Jung
  • D.-H. Park
  • S.-I. Chung
ORIGINAL PAPER

DOI: 10.1007/s002530050009

Cite this article as:
Lim, HK., Jung, KH., Park, DH. et al. Appl Microbiol Biotechnol (2000) 53: 201. doi:10.1007/s002530050009

Abstract

 Using high-cell-density culture of Escherichia coli under the control of an l-arabinose promoter (ParaB), several factors affecting the production of recombinant protein and the formation of inclusion bodies were studied. The inducer, l-arabinose, showed a maximal induction level above 10.7 mM in the final concentration. The concentration of inducer also affected the partition of interferon-α (IFN-α) into the soluble form and inclusion bodies. Induction kinetics of the rate of accumulation of IFN-α on the ParaB promoter showed a slower rate than those of other promoter systems, for example T7, lac or tac. These innate characteristics of ParaB enabled cells to grow continuously in spite of the metabolic burden induced by the expression of foreign protein. The duration time of induction could control the expression of both soluble and insoluble protein. The ratio of yeast extract to glycerol (N/C ratio) in feeding media significantly affected both the production level of recombinant protein and inclusion body formation. The reason for decreasing specific bioactivity during induction can be explained by the increased proportion of inclusion bodies in the total expressed IFN-α.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • H.-K. Lim
    • 1
  • K.-H. Jung
    • 1
  • D.-H. Park
    • 1
  • S.-I. Chung
    • 1
  1. 1.Mogam Biotechnology Research Institute, 341 Pojung-Ri Koosung-Myon, Yongin-City, Kyounggi-Do, 449-910, Korea Tel.: +82-331-2609800 Fax: +82-331-2609808 e-mail: khjung@kgcc.co.krKP