Applied Microbiology and Biotechnology

, Volume 54, Issue 2, pp 153–161

Ice crystallization by Pseudomonas syringae

  • N. Cochet
  • P. Widehem

DOI: 10.1007/s002530000377

Cite this article as:
Cochet, N. & Widehem, P. Appl Microbiol Biotechnol (2000) 54: 153. doi:10.1007/s002530000377


Several bacterial species can serve as biological ice nuclei. The best characterized of these is Pseudomonas syringae, a widely distributed bacterial epiphyte of plants. These biological ice nuclei find various applications in different fields, but an optimized production method was required in order to obtain the highly active cells which may be exploited as ice nucleators. The results presented here show that P. syringae cells reduce supercooling of liquid or solid media and enhance ice crystal formation at sub-zero temperatures, thus leading to a remarkable control of the crystallization phenomenon and a potential for energy savings. Our discussion focuses on recent and future applications of these ice nucleators in freezing operations, spray-ice technology and biotechnological processes.

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • N. Cochet
    • 1
  • P. Widehem
    • 1
  1. 1.Université de Technologie de Compiègne, Département Génie Biologique, BP 20, 529-60205 Compiègne, France e-mail: Tel.: +33-344-234454 Fax: +33-344-235276FR

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