Abstract
In this study, taro waste (TW) was utilized for Lactobacillus acidophilus BCRC 14079 cultivation and the anti-tumor and immune-modulatory properties of heat-killed cells (HKCs), cytoplasmic fraction (CF), and exopolysaccharide (EPS) were evaluated. The optimum liquefaction enzyme dosage, temperature, and time determined by Box-Behnken design response surface methodology (BBD-RSM) were 9 mL/L of α-amylase, 79.2 °C, and 5 h of reaction, respectively. The optimum temperature and reaction time for saccharification were determined as 60 °C and 3 h. The optimum medium, CGMY1 medium, constitutes of TW hydrolysate containing 37 g/L of glucose, 25 g/L of corn gluten meal (CGM), and 1 g/L of yeast extract (YE). Results of MTT assay showed that HKCs and EPS from CGM medium exhibited the highest anti-proliferative in HT-29 (IC50 of HKCs, 467.25 μg/mL; EPS, 716.10 μg/mL) and in Caco-2 cells (IC50 of EPS, 741.60 μg/mL). Luciferase-based NF-ΚB and COX-2 systems indicated HKCs from CGM medium stimulated the highest expression of luciferin in both systems. The luciferase activities by using 100 and 500 μg/mL of HKCs from CGM were 24.30- and 45.83-fold in NF-ΚB system and 11.54- and 4.93-fold in COX-2 system higher than the control. In conclusion, this study demonstrated the potential of TW medium for L. acidophilus cultivation and the production of non-viable probiotics with enhanced biological activities.
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This article was financially supported by the Ministry of Science and Technology, Taiwan, ROC (MOST 104-2221-E-002-125-MY3). The authors would like to thank Tien-Ni Tammy Tseng (who is a native speaker) from the Department of Agricultural Chemistry, National Taiwan University for the English editing.
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This study was funded by the Ministry of Science and Technology, Taiwan, ROC (MOST 104-2221-E-002-125-MY3). Shu-Chen Hsieh, Jui-Ming Liu, Xiao-Hui Pua, Yuwen Ting, Ren-Jun Hsu, and Kuan-Chen Cheng declare that he/she has no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors.
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Shu-Chen Hsieh and Jui-Ming Liu contributed equally to this work.
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Hsieh, SC., Liu, JM., Pua, XH. et al. Optimization of Lactobacillus acidophilus cultivation using taro waste and evaluation of its biological activity. Appl Microbiol Biotechnol 100, 2629–2639 (2016). https://doi.org/10.1007/s00253-015-7149-1
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DOI: https://doi.org/10.1007/s00253-015-7149-1