Applied Microbiology and Biotechnology

, Volume 99, Issue 20, pp 8777–8792

Effect of organic loading on the microbiota in a temperature-phased anaerobic digestion (TPAD) system co-digesting dairy manure and waste whey

  • Yueh-Fen Li
  • Christopher Abraham
  • Michael C. Nelson
  • Po-Hsu Chen
  • Joerg Graf
  • Zhongtang Yu
Environmental biotechnology

DOI: 10.1007/s00253-015-6738-3

Cite this article as:
Li, YF., Abraham, C., Nelson, M.C. et al. Appl Microbiol Biotechnol (2015) 99: 8777. doi:10.1007/s00253-015-6738-3

Abstract

Temperature-phased anaerobic digestion (TPAD) has gained increasing attention because it provides the flexibility to operate digesters under conditions that enhance overall digester performance. However, research on impact of organic overloading rate (OLR) to microbiota of TPAD systems was limited. In this study, we investigated the composition and successions of the microbiota in both the thermophilic and the mesophilic digesters of a laboratory-scale TPAD system co-digesting dairy manure and waste whey before and during organic overloading. The thermophilic and the mesophilic digesters were operated at 50 and 35 °C, respectively, with a hydraulic retention time (HRT) of 10 days for each digester. High OLR (dairy manure with 5 % total solid and waste whey of ≥60.4 g chemical oxygen demand (COD)/l/day) resulted in decrease in pH and in biogas production and accumulation of volatile fatty acids (VFAs) in the thermophilic digester, while the mesophilic digester remained unchanged except a transient increase in biogas production. Both denaturant gradient gel electrophoresis (DGGE) and Illumina sequencing of 16S ribosomal RNA (rRNA) gene amplicons showed dramatic change in microbiota composition and profound successions of both bacterial and methanogenic communities. During the overloading, Thermotogae was replaced by Proteobacteria, while Methanobrevibacter and archaeon classified as WCHD3-02 grew in predominance at the expense of Methanoculleus in the thermophilic digester, whereas Methanosarcina dominated the methanogenic community, while Methanobacterium and Methanobrevibacter became less predominant in the mesophilic digester. Canonical correspondence analysis (CCA) revealed that digester temperature and pH were the most influential environmental factors that explained much of the variations of the microbiota in this TPAD system when it was overloaded.

Keywords

DGGEIllumina sequencingMicrobiotaOrganic overloadingTPAD

Supplementary material

253_2015_6738_MOESM1_ESM.xlsx (148 kb)
Table S1(XLSX 147 kb)
253_2015_6738_MOESM2_ESM.pdf (34 kb)
Fig. S1(PDF 34 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Yueh-Fen Li
    • 1
  • Christopher Abraham
    • 2
  • Michael C. Nelson
    • 4
  • Po-Hsu Chen
    • 3
  • Joerg Graf
    • 4
  • Zhongtang Yu
    • 1
    • 2
  1. 1.Environmental Science Graduate ProgramThe Ohio State UniversityColumbusUSA
  2. 2.Department of Animal SciencesThe Ohio State UniversityColumbusUSA
  3. 3.Department of StatisticsThe Ohio State UniversityColumbusUSA
  4. 4.Department of Molecular and Cell BiologyUniversity of ConnecticutStorrsUSA