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Soil microbial community responses to antibiotic-contaminated manure under different soil moisture regimes

  • Environmental biotechnology
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Abstract

Sulfadiazine (SDZ) is an antibiotic frequently administered to livestock, and it alters microbial communities when entering soils with animal manure, but understanding the interactions of these effects to the prevailing climatic regime has eluded researchers. A climatic factor that strongly controls microbial activity is soil moisture. Here, we hypothesized that the effects of SDZ on soil microbial communities will be modulated depending on the soil moisture conditions. To test this hypothesis, we performed a 49-day fully controlled climate chamber pot experiments with soil grown with Dactylis glomerata (L.). Manure-amended pots without or with SDZ contamination were incubated under a dynamic moisture regime (DMR) with repeated drying and rewetting changes of >20 % maximum water holding capacity (WHCmax) in comparison to a control moisture regime (CMR) at an average soil moisture of 38 % WHCmax. We then monitored changes in SDZ concentration as well as in the phenotypic phospholipid fatty acid and genotypic 16S rRNA gene fragment patterns of the microbial community after 7, 20, 27, 34, and 49 days of incubation. The results showed that strongly changing water supply made SDZ accessible to mild extraction in the short term. As a result, and despite rather small SDZ effects on community structures, the PLFA-derived microbial biomass was suppressed in the SDZ-contaminated DMR soils relative to the CMR ones, indicating that dynamic moisture changes accelerate the susceptibility of the soil microbial community to antibiotics.

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Acknowledgments

We thank Elvira Sieberger and Petra Ziegler for the practical support. This project was funded by the German Research Foundation (DFG) within the Research Unit FOR 566 “Veterinary medicines in soil: basic research for risk assessment.”

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Authors and Affiliations

  1. Soil Science, Faculty of Regional and Environmental Sciences, University of Trier, Behringstraße 21, 54286, Trier, Germany

    Rüdiger Reichel & Sören Thiele-Bruhn

  2. Research Unit for Environmental Genomics, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstraße 1, 85764, Oberschleißheim, Germany

    Viviane Radl & Michael Schloter

  3. Institute of Crop Science and Resource Conservation (INRES), Soil Science and Soil Ecology, University of Bonn, Nussallee 13, 53115, Bonn, Germany

    Ingrid Rosendahl & Wulf Amelung

  4. Research Unit for Environmental Simulation, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Ingolstädter Landstraße 1, 85764, Oberschleißheim, Germany

    Andreas Albert

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  1. Rüdiger Reichel
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  2. Viviane Radl
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Correspondence to Sören Thiele-Bruhn.

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Reichel, R., Radl, V., Rosendahl, I. et al. Soil microbial community responses to antibiotic-contaminated manure under different soil moisture regimes. Appl Microbiol Biotechnol 98, 6487–6495 (2014). https://doi.org/10.1007/s00253-014-5717-4

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  • DOI: https://doi.org/10.1007/s00253-014-5717-4

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