Applied Microbiology and Biotechnology

, Volume 98, Issue 8, pp 3413–3424

Small RNA regulators in bacteria: powerful tools for metabolic engineering and synthetic biology

Mini-Review

DOI: 10.1007/s00253-014-5569-y

Cite this article as:
Kang, Z., Zhang, C., Zhang, J. et al. Appl Microbiol Biotechnol (2014) 98: 3413. doi:10.1007/s00253-014-5569-y

Abstract

Small RNAs, a large class of ancient posttranscriptional regulators, have recently attracted considerable attention. A plethora of small RNAs has been identified and characterized, many of which belong to the major small noncoding RNA (sRNA) or riboswitch families. It has become increasingly clear that most small RNAs play critical regulatory roles in many processes and are, therefore, considered to be powerful tools for metabolic engineering and synthetic biology. In this review, we describe recent achievements in the identification, characterization, and application of small RNAs. We give particular attention to advances in the design and synthesis of novel sRNAs and riboswitches for metabolic engineering. In addition, a novel strategy for hierarchical control of global metabolic pathways is proposed.

Keywords

Small RNARiboswitchGene expressionPosttranscriptional regulationMetabolic engineeringSynthetic biology

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Zhen Kang
    • 1
    • 2
    • 4
    • 6
  • Chuanzhi Zhang
    • 2
  • Junli Zhang
    • 2
  • Peng Jin
    • 2
  • Juan Zhang
    • 1
    • 2
    • 4
  • Guocheng Du
    • 1
    • 2
    • 3
    • 4
  • Jian Chen
    • 1
    • 2
    • 4
    • 5
  1. 1.Synergetic Innovation Center of Food Safety and NutritionWuxiChina
  2. 2.The Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan UniversityWuxiChina
  3. 3.The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of EducationJiangnan UniversityWuxiChina
  4. 4.Synergy Innovation Center of Modern Industrial FermentationWuxiChina
  5. 5.National Engineering Laboratory for Cereal Fermentation TechnologyJiangnan UniversityWuxiChina
  6. 6.Yixing Union Biochemical Co., Ltd.WuxiChina