Mini-Review

Applied Microbiology and Biotechnology

, Volume 98, Issue 8, pp 3413-3424

Small RNA regulators in bacteria: powerful tools for metabolic engineering and synthetic biology

  • Zhen KangAffiliated withSynergetic Innovation Center of Food Safety and NutritionThe Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan UniversitySynergy Innovation Center of Modern Industrial FermentationYixing Union Biochemical Co., Ltd. Email author 
  • , Chuanzhi ZhangAffiliated withThe Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University
  • , Junli ZhangAffiliated withThe Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University
  • , Peng JinAffiliated withThe Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University
  • , Juan ZhangAffiliated withSynergetic Innovation Center of Food Safety and NutritionThe Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan UniversitySynergy Innovation Center of Modern Industrial Fermentation
  • , Guocheng DuAffiliated withSynergetic Innovation Center of Food Safety and NutritionThe Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan UniversityThe Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan UniversitySynergy Innovation Center of Modern Industrial Fermentation
  • , Jian ChenAffiliated withSynergetic Innovation Center of Food Safety and NutritionThe Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan UniversitySynergy Innovation Center of Modern Industrial FermentationNational Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Small RNAs, a large class of ancient posttranscriptional regulators, have recently attracted considerable attention. A plethora of small RNAs has been identified and characterized, many of which belong to the major small noncoding RNA (sRNA) or riboswitch families. It has become increasingly clear that most small RNAs play critical regulatory roles in many processes and are, therefore, considered to be powerful tools for metabolic engineering and synthetic biology. In this review, we describe recent achievements in the identification, characterization, and application of small RNAs. We give particular attention to advances in the design and synthesis of novel sRNAs and riboswitches for metabolic engineering. In addition, a novel strategy for hierarchical control of global metabolic pathways is proposed.

Keywords

Small RNA Riboswitch Gene expression Posttranscriptional regulation Metabolic engineering Synthetic biology