Applied Microbiology and Biotechnology

, Volume 97, Issue 24, pp 10255–10262

ω3 fatty acid desaturases from microorganisms: structure, function, evolution, and biotechnological use

  • Mingxuan Wang
  • Haiqin Chen
  • Zhennan Gu
  • Hao Zhang
  • Wei Chen
  • Yong Q. Chen
Mini-Review

DOI: 10.1007/s00253-013-5336-5

Cite this article as:
Wang, M., Chen, H., Gu, Z. et al. Appl Microbiol Biotechnol (2013) 97: 10255. doi:10.1007/s00253-013-5336-5
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Abstract

The biosynthesis of very-long-chain polyunsaturated fatty acids involves an alternating process of fatty acid desaturation and elongation catalyzed by complex series of enzymes. ω3 desaturase plays an important role in converting ω6 fatty acids into ω3 fatty acids. Genes for this desaturase have been identified and characterized in a wide range of microorganisms, including cyanobacteria, yeasts, molds, and microalgae. Like all fatty acid desaturases, ω3 desaturase is structurally characterized by the presence of three highly conserved histidine-rich motifs; however, unlike some desaturases, it lacks a cytochrome b5-like domain. Understanding the structure, function, and evolution of ω3 desaturases, particularly their substrate specificities in the biosynthesis of very-long-chain polyunsaturated fatty acids, lays the foundation for potential production of various ω3 fatty acids in transgenic microorganisms.

Keywords

Polyunsaturated fatty acids (PUFAs) ω3 desaturase Lipid biochemistry Substrate specificity Biotechnology 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Mingxuan Wang
    • 1
  • Haiqin Chen
    • 1
    • 2
  • Zhennan Gu
    • 1
    • 2
  • Hao Zhang
    • 1
    • 2
  • Wei Chen
    • 1
    • 2
  • Yong Q. Chen
    • 1
    • 2
    • 3
  1. 1.State Key Laboratory of Food Science and Technology, School of Food Science and TechnologyJiangnan UniversityWuxiPeople’s Republic of China
  2. 2.Synergetic Innovation Center of Food Safety and NutritionWuxiPeople’s Republic of China
  3. 3.Department of Cancer BiologyWake Forest School of MedicineWinston-SalemUSA

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