Applied microbial and cell physiology

Applied Microbiology and Biotechnology

, Volume 97, Issue 18, pp 8273-8281

First online:

Cholesterol-lowering probiotics: in vitro selection and in vivo testing of bifidobacteria

  • Alessandra BordoniAffiliated withDepartment of Agro-Food Sciences and Technologies, University of Bologna
  • , Alberto AmarettiAffiliated withDepartment of Life Sciences, University of Modena and Reggio Emilia
  • , Alan LeonardiAffiliated withDepartment of Life Sciences, University of Modena and Reggio Emilia
  • , Elisa BoschettiAffiliated withDepartment of Biomedical and Neuromotor Science, University of Bologna
  • , Francesca DanesiAffiliated withDepartment of Agro-Food Sciences and Technologies, University of Bologna
  • , Diego MatteuzziAffiliated withDepartment of Pharmaceutical Sciences, University of Bologna
  • , Lucia RoncagliaAffiliated withDepartment of Life Sciences, University of Modena and Reggio Emilia
  • , Stefano RaimondiAffiliated withDepartment of Life Sciences, University of Modena and Reggio Emilia
  • , Maddalena RossiAffiliated withDepartment of Life Sciences, University of Modena and Reggio Emilia Email author 

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Abstract

Thirty-four strains of bifidobacteria belonging to Bifidobacterium adolescentis, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, and Bifidobacterium pseu-docatenulatum were assayed in vitro for the ability to assimilate cholesterol and for bile salt hydrolase (BSH) against glycocholic and taurodeoxycholic acids (GCA and TDCA). Cholesterol assimilation was peculiar characteristic of two strains belonging to the species B. bifidum (B. bifidum MB 107 and B. bifidum MB 109), which removed 81 and 50 mg of cholesterol per gram of biomass, being the median of specific cholesterol absorption by bifidobacteria 19 mg/g. Significant differences in BSH activities were not established among bifidobacterial species. However, the screening resulted in the selection of promising strains able to efficiently deconjugate GCA and TDCA. No relationship was recognized between BSH phenotype and the extent of cholesterol assimilation. On the basis of cholesterol assimilation or BSHGCA and BSHTDCA activities, B. bifidum MB 109 (DSMZ 23731), B. breve MB 113 (DSMZ 23732), and B. animalis subsp. lactis MB 2409 (DSMZ 23733) were combined in a probiotic mixture to be fed to hypercholesterolemic rats. The administration of this probiotic formulation resulted in a significant reduction of total cholesterol and low-density cholesterol (LDL-C), whereas it did not affect high-density cholesterol (HDL-C) and HDL-C/LDL-C ratio.

Keywords

Bifidobacterium Probiotic Cholesterol Bile salt hydrolase In vivo In vitro