Applied Microbiology and Biotechnology

, Volume 97, Issue 10, pp 4315–4323

Role of the pentose phosphate pathway and the Entner–Doudoroff pathway in glucose metabolism of Gluconobacter oxydans 621H

Biotechnological products and process engineering

DOI: 10.1007/s00253-013-4707-2

Cite this article as:
Richhardt, J., Bringer, S. & Bott, M. Appl Microbiol Biotechnol (2013) 97: 4315. doi:10.1007/s00253-013-4707-2

Abstract

Glucose catabolism by the obligatory aerobic acetic acid bacterium Gluconobacter oxydans 621H proceeds in two phases comprising rapid periplasmic oxidation of glucose to gluconate (phase I) and oxidation of gluconate to 2-ketogluconate or 5-ketogluconate (phase II). Only a small amount of glucose and part of the gluconate is taken up into the cells. To determine the roles of the pentose phosphate pathway (PPP) and the Entner–Doudoroff pathway (EDP) for intracellular glucose and gluconate catabolism, mutants defective in either the PPP (Δgnd, Δgnd zwf*) or the EDP (Δedd–eda) were characterized under defined conditions of pH 6 and 15 % dissolved oxygen. In the presence of yeast extract, neither of the two pathways was essential for growth with glucose. However, the PPP mutants showed a reduced growth rate in phase I and completely lacked growth in phase II. In contrast, the EDP mutant showed the same growth behavior as the reference strain. These results demonstrate that the PPP is of major importance for cytoplasmic glucose and gluconate catabolism, whereas the EDP is dispensable. Reasons for this difference are discussed.

Keywords

Gluconobacter oxydansgndzwfeddedaGlucose

Supplementary material

253_2013_4707_MOESM1_ESM.docx (105 kb)
ESM 1(DOCX 104 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Institut für Bio- und Geowissenschaften, IBG-1: BiotechnologieForschungszentrum JülichJülichGermany