Environmental biotechnology

Applied Microbiology and Biotechnology

, Volume 97, Issue 20, pp 9257-9262

Selective bactericidal activity of nanopatterned superhydrophobic cicada Psaltoda claripennis wing surfaces

  • Jafar HasanAffiliated withFaculty Life and Social Sciences, Swinburne University of Technology
  • , Hayden K. WebbAffiliated withFaculty Life and Social Sciences, Swinburne University of Technology
  • , Vi Khanh TruongAffiliated withFaculty Life and Social Sciences, Swinburne University of Technology
  • , Sergey PogodinAffiliated withDepartament d’Enginyeria Quimica, Universitat Rovira i Virgili
  • , Vladimir A. BaulinAffiliated withDepartament d’Enginyeria Quimica, Universitat Rovira i VirgiliICREA
  • , Gregory S. WatsonAffiliated withSchool of Marine and Biological Sciences, James Cook University
  • , Jolanta A. WatsonAffiliated withSchool of Marine and Biological Sciences, James Cook University
  • , Russell J. CrawfordAffiliated withFaculty Life and Social Sciences, Swinburne University of Technology
  • , Elena P. IvanovaAffiliated withFaculty Life and Social Sciences, Swinburne University of Technology Email author 

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Abstract

The nanopattern on the surface of Clanger cicada (Psaltoda claripennis) wings represents the first example of a new class of biomaterials that can kill bacteria on contact based solely on its physical surface structure. As such, they provide a model for the development of novel functional surfaces that possess an increased resistance to bacterial contamination and infection. Their effectiveness against a wide spectrum of bacteria, however, is yet to be established. Here, the bactericidal properties of the wings were tested against several bacterial species, possessing a range of combinations of morphology and cell wall type. The tested species were primarily pathogens, and included Bacillus subtilis, Branhamella catarrhalis, Escherichia coli, Planococcus maritimus, Pseudomonas aeruginosa, Pseudomonas fluorescens, and Staphylococcus aureus. The wings were found to consistently kill Gram-negative cells (i.e., B. catarrhalis, E. coli, P. aeruginosa, and P. fluorescens), while Gram-positive cells (B. subtilis, P. maritimus, and S. aureus) remained resistant. The morphology of the cells did not appear to play any role in determining cell susceptibility. The bactericidal activity of the wing was also found to be quite efficient; 6.1 ± 1.5 × 106 P. aeruginosa cells in suspension were inactivated per square centimeter of wing surface after 30-min incubation. These findings demonstrate the potential for the development of selective bactericidal surfaces incorporating cicada wing nanopatterns into the design.

Keywords

Self-cleaning Nanopattern Bactericidal Insect wings Antibiofouling