Biotechnological products and process engineering

Applied Microbiology and Biotechnology

, Volume 97, Issue 8, pp 3373-3381

First online:

Adaptation of the Spodoptera exigua Se301 insect cell line to grow in serum-free suspended culture. Comparison of SeMNPV productivity in serum-free and serum-containing media

  • A. Beas-CatenaAffiliated withDepartment of Chemical Engineering, University of Almeria
  • , A. Sánchez-MirónAffiliated withDepartment of Chemical Engineering, University of Almeria Email author 
  • , F. García-CamachoAffiliated withDepartment of Chemical Engineering, University of Almeria
  • , A. Contreras-GómezAffiliated withDepartment of Chemical Engineering, University of Almeria
  • , E. Molina-GrimaAffiliated withDepartment of Chemical Engineering, University of Almeria

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Abstract

Spodoptera exigua Se301 cells have been successfully adapted to two different commercial serum-free media (SFM; Ex-Cell 420 and Serum-Free Insect Medium-1) by gradually reducing the 10 %-added serum-containing medium content from 100 % to 0 % (v/v) in suspended cultures. Both direct adaptation to a serum-free medium and cell growth in the absence of protective additives against fluid dynamic stress [polyvinyl pyrrolidone and polyvinyl alcohol] and disaggregation [dextran sulfate] proved impossible. Cells grew reproducibly in both SFMs once the serum had been completely removed, although the use of Ex-Cell 420 resulted in higher growth rates and cell densities. Turbulence was sufficiently high to reduce growth rates and final cell densities at the highest Reynolds number investigated, although no clear influence of agitation was observed on virus productivity. Both attached and suspended Se301 cell cultures were successfully infected with the SeMNPV baculovirus. Cells adapted to different conditions (attached or suspended culture, serum-containing or serum-free medium) showed different occlusion bodies productivities at the two multiplicities of infection assayed (0.1 and 0.5).

Keywords

Spodoptera exigua Baculovirus Serum-free medium Suspension culture Cell damage Shear stress