Applied Microbiology and Biotechnology

, Volume 96, Issue 4, pp 1079–1091

An efficient xylose-fermenting recombinant Saccharomyces cerevisiae strain obtained through adaptive evolution and its global transcription profile

  • Yu Shen
  • Xiao Chen
  • Bingyin Peng
  • Liyuan Chen
  • Jin Hou
  • Xiaoming Bao
Bioenergy and biofuels

DOI: 10.1007/s00253-012-4418-0

Cite this article as:
Shen, Y., Chen, X., Peng, B. et al. Appl Microbiol Biotechnol (2012) 96: 1079. doi:10.1007/s00253-012-4418-0

Abstract

Factors related to ethanol production from xylose in engineered Saccharomyces cerevisiae that contain an exogenous initial metabolic pathway are still to be elucidated. In the present study, a strain that expresses the xylose isomerase gene of Piromyces sp. Pi-xylA and overexpresses XKS1, RPE1, RKI1, TAL1, and TKL1, with deleted GRE3 and COX4 genes was constructed. The xylose utilization capacity of the respiratory deficiency strain was poor but improved via adaptive evolution in xylose. The μmax of the evolved strain in 20 g l−1 xylose is 0.11 ± 0.00 h−1, and the evolved strain consumed 17.83 g l−1 xylose within 72 h, with an ethanol yield of 0.43 g g−1 total consumed sugars during glucose–xylose cofermentation. Global transcriptional changes and effect of several specific genes were studied. The result revealed that the increased xylose isomerase acivity, the upregulation of enzymes involved in glycolysis and glutamate synthesis, and the downregulation of trehalose and glycogen synthesis, may have contributed to the improved xylose utilization of the strain. Furthermore, the deletion of PHO13 decreased the xylose growth in the respiration deficiency strain although deleting PHO13 can improve the xylose metabolism in other strains.

Keywords

Xylose isomerase Respiratory deficiency Ethanol PDC6 PHO13 CWP1 

Supplementary material

253_2012_4418_MOESM1_ESM.docx (314 kb)
ESM 1(DOCX 314 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Yu Shen
    • 1
  • Xiao Chen
    • 1
  • Bingyin Peng
    • 1
  • Liyuan Chen
    • 1
  • Jin Hou
    • 1
  • Xiaoming Bao
    • 1
  1. 1.The State Key Laboratory of Microbial TechnologyShandong UniversityJinanChina

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