Applied Microbiology and Biotechnology

, Volume 97, Issue 5, pp 2109–2118

Effects of Lactobacillus salivarius, Lactobacillus reuteri, and Pediococcus acidilactici on the nematode Caenorhabditis elegans include possible antitumor activity

  • Michael K. Fasseas
  • Costas Fasseas
  • Konstantinos C. Mountzouris
  • Popi Syntichaki
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-012-4357-9

Cite this article as:
Fasseas, M.K., Fasseas, C., Mountzouris, K.C. et al. Appl Microbiol Biotechnol (2013) 97: 2109. doi:10.1007/s00253-012-4357-9

Abstract

This study examined the effects of three lactic acid bacteria (LAB) strains on the nematode Caenorhabditis elegans. Lactobacillus salivarius, Lactobacillus reuteri, and Pediococcus acidilactici were found to inhibit the development and growth of the worm. Compared to Escherichia coli used as the control, L. reuteri and P. acidilactici reduced the lifespan of wild-type and short-lived daf-16 worms. On the contrary, L. salivarius extended the lifespan of daf-16 worms when used live, but reduced it as UV-killed bacteria. The three LAB induced the expression of genes involved in pathogen response and inhibited the growth of tumor-like germ cells, without affecting DAF16 localization or increasing corpse cells. Our results suggest the possible use of C. elegans as a model for studying the antitumor attributes of LAB. The negative effects of these LAB strains on the nematode also indicate their potential use against parasitic nematodes.

Keywords

Lactic acid bacteriaProbioticC. elegansAntitumorImmune response

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Michael K. Fasseas
    • 1
  • Costas Fasseas
    • 2
  • Konstantinos C. Mountzouris
    • 3
  • Popi Syntichaki
    • 1
  1. 1.Center of Basic Research IIBiomedical Research Foundation of the Academy of AthensAthensGreece
  2. 2.Department of Agricultural BiotechnologyAgricultural University of AthensAthensGreece
  3. 3.Department of Nutritional Physiology and Feeding, Faculty of Animal Science and AquacultureAgricultural University of AthensAthensGreece