Applied Microbiology and Biotechnology

, Volume 97, Issue 2, pp 775–782

Biogenic antimicrobial silver nanoparticles produced by fungi

  • Alexandre G. Rodrigues
  • Liu Yu Ping
  • Priscyla D. Marcato
  • Oswaldo L. Alves
  • Maria C. P. Silva
  • Rita C. Ruiz
  • Itamar S. Melo
  • Ljubica Tasic
  • Ana O. De Souza
Applied microbial and cell physiology

DOI: 10.1007/s00253-012-4209-7

Cite this article as:
Rodrigues, A.G., Ping, L.Y., Marcato, P.D. et al. Appl Microbiol Biotechnol (2013) 97: 775. doi:10.1007/s00253-012-4209-7

Abstract

Aspergillus tubingensis and Bionectria ochroleuca showed excellent extracellular ability to synthesize silver nanoparticles (Ag NP), spherical in shape and 35 ± 10 nm in size. Ag NP were characterized by transmission electron microscopy, X-ray diffraction analysis, and photon correlation spectroscopy for particle size and zeta potential. Proteins present in the fungal filtrate and in Ag NP dispersion were analyzed by electrophoresis (sodium dodecyl sulfate polyacrylamide gel electrophoresis). Ag NP showed pronounced antifungal activity against Candida sp, frequently occurring in hospital infections, with minimal inhibitory concentration in the range of 0.11–1.75 μg/mL. Regarding antibacterial activity, nanoparticles produced by A. tubingensis were more effective compared to the other fungus, inhibiting 98.0 % of Pseudomonas. aeruginosa growth at 0.28 μg/mL. A. tubingensis synthesized Ag NP with surprisingly high and positive surface potential, differing greatly from all known fungi. These data open the possibility of obtaining biogenic Ag NP with positive surface potential and new applications.

Keywords

Silver nanoparticles Antimicrobial activity Aspergillus tubingensis Bionectria ochroleuca Mangrove 

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Alexandre G. Rodrigues
    • 1
  • Liu Yu Ping
    • 1
  • Priscyla D. Marcato
    • 2
  • Oswaldo L. Alves
    • 2
  • Maria C. P. Silva
    • 3
  • Rita C. Ruiz
    • 4
  • Itamar S. Melo
    • 5
  • Ljubica Tasic
    • 2
  • Ana O. De Souza
    • 1
  1. 1.Laboratório de Bioquímica e BiofísicaInstituto ButantanSão PauloBrazil
  2. 2.IQUniversidade Estadual de CampinasSão PauloBrazil
  3. 3.ICBUniversidade de São PauloSão PauloBrazil
  4. 4.Laboratório de BacteriologiaInstituto ButantanSão PauloBrazil
  5. 5.Embrapa Meio AmbienteSão PauloBrazil

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