Applied Microbiology and Biotechnology

, Volume 94, Issue 4, pp 939–948

Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation

  • Akihito Nakanishi
  • Jun Gu Bae
  • Kotaro Fukai
  • Naoki Tokumoto
  • Kouichi Kuroda
  • Jun Ogawa
  • Masato Nakatani
  • Sakayu Shimizu
  • Mitsuyoshi Ueda
Biotechnological products and process engineering

DOI: 10.1007/s00253-012-3876-8

Cite this article as:
Nakanishi, A., Bae, J.G., Fukai, K. et al. Appl Microbiol Biotechnol (2012) 94: 939. doi:10.1007/s00253-012-3876-8

Abstract

A gene encoding laccase I was identified and cloned from the white-rot fungus Trametes sp. Ha1. Laccase I contained 10 introns and an original secretion signal sequence. After laccase I without introns was prepared by overlapping polymerase chain reaction, it was inserted into expression vector pULD1 for yeast cell surface display. The oxidation activity of a laccase-I-displaying yeast as a whole-cell biocatalyst was examined with 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and the constructed yeast showed a high oxidation activity. After the pretreatment of hydrothermally processed rice straw (HPRS) with laccase-I-displaying yeast with ABTS, fermentation was conducted with yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase with HPRS. Fermentation of HPRS treated with laccase-I-displaying yeast was performed with 1.21-fold higher activities than those of HPRS treated with control yeast. The results indicated that pretreatment with laccase-I-displaying yeast with ABTS was effective for direct fermentation of cellulosic materials by yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase.

Keywords

BiorefineryLaccaseCell surface engineering of yeastTrametes sp.Lignin

Supplementary material

253_2012_3876_MOESM1_ESM.doc (189 kb)
ESM 1(DOC 189 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Akihito Nakanishi
    • 1
  • Jun Gu Bae
    • 1
  • Kotaro Fukai
    • 1
  • Naoki Tokumoto
    • 1
  • Kouichi Kuroda
    • 1
  • Jun Ogawa
    • 1
  • Masato Nakatani
    • 2
  • Sakayu Shimizu
    • 1
  • Mitsuyoshi Ueda
    • 1
  1. 1.Division of Applied Life Sciences, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.Daiwa KaseiShigaJapan