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Long-term field fertilization alters the diversity of autotrophic bacteria based on the ribulose-1,5-biphosphate carboxylase/oxygenase (RubisCO) large-subunit genes in paddy soil

  • Environmental biotechnology
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Abstract

Carbon dioxide (CO2) assimilation by autotrophic bacteria is an important process in the soil carbon cycle with major environmental implications. The long-term impact of fertilizer on CO2 assimilation in the bacterial community of paddy soils remains poorly understood. To narrow this knowledge gap, the composition and abundance of CO2-assimilating bacteria were investigated using terminal restriction fragment length polymorphism and quantitative PCR of the cbbL gene [that encodes ribulose-1,5-biphosphate carboxylase/oxygenase (RubisCO)] in paddy soils. Soils from three stations in subtropical China were used. Each station is part of a long-term fertilization experiment with three treatments: no fertilizer (CK), chemical fertilizers (NPK), and NPK combined with rice straw (NPKM). At all of the stations, the cbbL-containing bacterial communities were dominated by facultative autotrophic bacteria such as Rhodopseudomonas palustris, Bradyrhizobium japonicum, and Ralstonia eutropha. The community composition in the fertilized soil (NPK and NPKM) was distinct from that in unfertilized soil (CK). The bacterial cbbL abundance (3–8 × 108 copies g soil−1) and RubisCO activity (0.40–1.76 nmol CO2 g soil−1 min−1) in paddy soils were significantly positively correlated, and both increased with the addition of fertilizer. Among the measured soil parameters, soil organic carbon and pH were the most significant factors influencing the community composition, abundance, and activity of the cbbL-containing bacteria. These results suggest that long-term fertilization has a strong impact on the activity and community of cbbL-containing bacterial populations in paddy soils, especially when straw is combined with chemical fertilizers.

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Acknowledgments

This work was financially supported by funds from the Chinese Academy of Sciences (grant no. KZCX2-YW-T07), the National Natural Science Foundation of China (grant no. 41090283, 40901124), and the Knowledge Innovation Program of the Chinese Academy of Sciences (grant no. ISACX-LYQY-QN-1103).

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Authors and Affiliations

  1. Key Laboratory of Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, 410125, China

    Hongzhao Yuan, Tida Ge, Xiaohong Wu, Shoulong Liu, Chengli Tong, Hongling Qin, Wenxue Wei & Jinshui Wu

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Hongzhao Yuan

  3. Xinxiang Medical University, Xinxiang, Henan, 450003, China

    Minna Wu

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  1. Hongzhao Yuan
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  2. Tida Ge
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  3. Xiaohong Wu
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  5. Chengli Tong
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  6. Hongling Qin
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  7. Minna Wu
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  9. Jinshui Wu
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Correspondence to Jinshui Wu.

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Hongzhao Yuan and Tida Ge contributed equally to this work as co-first authors.

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Yuan, H., Ge, T., Wu, X. et al. Long-term field fertilization alters the diversity of autotrophic bacteria based on the ribulose-1,5-biphosphate carboxylase/oxygenase (RubisCO) large-subunit genes in paddy soil. Appl Microbiol Biotechnol 95, 1061–1071 (2012). https://doi.org/10.1007/s00253-011-3760-y

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  • DOI: https://doi.org/10.1007/s00253-011-3760-y

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