Applied Microbiology and Biotechnology

, Volume 93, Issue 5, pp 1917–1925

Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production

  • Yuyang Song
  • Ken’ichiro Matsumoto
  • Miwa Yamada
  • Aoi Gohda
  • Christopher J. Brigham
  • Anthony J. Sinskey
  • Seiichi Taguchi
Biotechnological products and process engineering

DOI: 10.1007/s00253-011-3718-0

Cite this article as:
Song, Y., Matsumoto, K., Yamada, M. et al. Appl Microbiol Biotechnol (2012) 93: 1917. doi:10.1007/s00253-011-3718-0
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Abstract

The first biosynthetic system for lactate (LA)-based polyesters was previously created in recombinant Escherichia coli (Taguchi et al. 2008). Here, we have begun efforts to upgrade the prototype polymer production system to a practical stage by using metabolically engineered Gram-positive bacterium Corynebacterium glutamicum as an endotoxin-free platform. We designed metabolic pathways in C. glutamicum to generate monomer substrates, lactyl-CoA (LA-CoA), and 3-hydroxybutyryl-CoA (3HB-CoA), for the copolymerization catalyzed by the LA-polymerizing enzyme (LPE). LA-CoA was synthesized by D-lactate dehydrogenase and propionyl-CoA transferase, while 3HB-CoA was supplied by β-ketothiolase (PhaA) and NADPH-dependent acetoacetyl-CoA reductase (PhaB). The functional expression of these enzymes led to a production of P(LA-co-3HB) with high LA fractions (96.8 mol%). The omission of PhaA and PhaB from this pathway led to a further increase in LA fraction up to 99.3 mol%. The newly engineered C. glutamicum potentially serves as a food-grade and biomedically applicable platform for the production of poly(lactic acid)-like polyester.

Keywords

Polylactide Biobased plastic PHA synthase Polyhydroxyalkanoate Polyhydroxybutyrate 

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yuyang Song
    • 1
  • Ken’ichiro Matsumoto
    • 1
  • Miwa Yamada
    • 1
    • 2
  • Aoi Gohda
    • 1
  • Christopher J. Brigham
    • 3
  • Anthony J. Sinskey
    • 3
  • Seiichi Taguchi
    • 1
  1. 1.Division of Biotechnology and Macromolecular ChemistryGraduate School of Engineering, Hokkaido UniversitySapporoJapan
  2. 2.Department of Biological Chemistry and Food ScienceIwate UniversityMoriokaJapan
  3. 3.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA

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