Applied Microbiology and Biotechnology

, Volume 93, Issue 3, pp 1207–1219

The effect of scale on gene expression: commercial versus laboratory wine fermentations

  • Debra Rossouw
  • Neil Jolly
  • Dan Jacobson
  • Florian F. Bauer
Original Paper

DOI: 10.1007/s00253-011-3564-0

Cite this article as:
Rossouw, D., Jolly, N., Jacobson, D. et al. Appl Microbiol Biotechnol (2012) 93: 1207. doi:10.1007/s00253-011-3564-0

Abstract

Molecular and cellular processes that are responsible for industrially relevant phenotypes of fermenting microorganisms are a central focus of biotechnological research. Such research intends to generate insights and solutions for fermentation-based industries with regards to issues such as improving product yield or the quality of the final fermentation product. For logistical reasons, and to ensure data reproducibility, such research is mostly carried out in defined or synthetic media and in small-scale fermentation vessels. Two questions are frequently raised regarding the applicability of this approach to solve problems experienced in industrial fermentations: (1) Is synthetic medium a sufficiently accurate approximation of the generally more complex natural (and frequently highly variable) substrates that are employed in most fermentation-based industries, and (2) can results obtained in small-scale laboratory fermentations be extrapolated to large-scale industrial environments? Here, we address the second question through a comparative transcriptomic approach by assessing the response of an industrial wine yeast strain fermenting a natural grape juice in small-scale laboratory and large-scale industrial conditions. In yeast, transcriptome analysis is arguably the best available tool to holistically assess the physiological state of a population and its response to changing environmental conditions. The data suggest that scale does indeed impact on some environmental parameters such as oxygen availability. However, the data show that small-scale fermentations nevertheless accurately reflect general molecular processes and adaptations during large-scale fermentation and that extrapolation of laboratory datasets to real industrial processes can be justified.

Keywords

TranscriptomicsScaleCommercial wine fermentation

Supplementary material

253_2011_3564_MOESM1_ESM.docx (68 kb)
ESMdocx (DOCX 67 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Debra Rossouw
    • 1
  • Neil Jolly
    • 2
  • Dan Jacobson
    • 1
  • Florian F. Bauer
    • 1
  1. 1.Institute for Wine BiotechnologyUniversity of StellenboschStellenboschSouth Africa
  2. 2.ARC Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute of the Agricultural Research Council)StellenboschSouth Africa