Applied Microbiology and Biotechnology

, Volume 93, Issue 2, pp 777–785

The cytotoxicity and anticancer mechanisms of alterporriol L, a marine bianthraquinone, against MCF-7 human breast cancer cells

  • Caihuan Huang
  • Hua Jin
  • Bing Song
  • Xun Zhu
  • Hongxia Zhao
  • Jiye Cai
  • Yongjun Lu
  • Bin Chen
  • Yongcheng Lin
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-011-3463-4

Cite this article as:
Huang, C., Jin, H., Song, B. et al. Appl Microbiol Biotechnol (2012) 93: 777. doi:10.1007/s00253-011-3463-4

Abstract

Alterporriol L, a new bianthraquinone derivative, was isolated from a marine fungus Alternaria sp. ZJ9-6B. The cytotoxic activity and anticancer mechanisms of alterporriol L towards breast cancer cells lines were detected using MTT assay, immunofluorescence, and flow cytometry. Simultaneously, the changes in morphological properties of cells were detected before and after treatment with alterporriol L by atomic force microscope (AFM) at a nanometer scale. MTT assay showed that alterporriol L could effectively inhibit the growth and proliferation, and there was a dose-dependent manner of cell death. Moreover, the alterporriol L could induce cancer cell apoptosis or necrosis. Furthermore, the reactive oxygen species, mitochondrial membrane potential, and cytosolic free calcium level were changed after treatment with alterporriol L, suggesting that alterporriol L played vital roles in breast cancer cells through destroying the mitochondrial. And all these alterations are in accord with changes of morphology detected by AFM, which suggested that the AFM is a useful tool to detect the morphological changes of the cancer cells.

Keywords

Marine bianthraquinone Breast cancer Apoptosis Atomic force microscopy 

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Caihuan Huang
    • 1
    • 2
    • 3
  • Hua Jin
    • 4
  • Bing Song
    • 5
  • Xun Zhu
    • 2
  • Hongxia Zhao
    • 6
  • Jiye Cai
    • 4
  • Yongjun Lu
    • 2
  • Bin Chen
    • 1
    • 2
  • Yongcheng Lin
    • 1
    • 2
  1. 1.School of Chemistry and Chemical EngineeringSun Yat-sen UniversityGuangzhouChina
  2. 2.Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganism (Sun Yat-sen University)Bureau of Education of GuangdongGuangzhouChina
  3. 3.School of Science and EngineeringJinan UniversityGuangzhouChina
  4. 4.Department of ChemistryJinan UniversityGuangzhouChina
  5. 5.The Institute for Tissue Transplantation and ImmunologyJinan UniversityGuangzhouChina
  6. 6.Faculty of Chemical Engineering and Light IndustryGuangdong University of TechnologyGuangzhouChina

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