Applied Microbiology and Biotechnology

, Volume 91, Issue 6, pp 1623–1632

Effects of nitrogen sources on production and composition of sophorolipids by Wickerhamiella domercqiae var. sophorolipid CGMCC 1576

  • Xiao-jing Ma
  • Hui Li
  • Ling-jian Shao
  • Jing Shen
  • Xin Song
Applied Microbial and Cell Physiology

DOI: 10.1007/s00253-011-3327-y

Cite this article as:
Ma, X., Li, H., Shao, L. et al. Appl Microbiol Biotechnol (2011) 91: 1623. doi:10.1007/s00253-011-3327-y

Abstract

The effects of nitrogen sources on growth of sophorolipid-producing yeast, Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 and on production and composition of sophorolipids were studied. Organic nitrogen sources are more favorable for accumulation of biomass than inorganic ones. Presence of ammonium ion from different inorganic nitrogen sources (except NH4HCO3) greatly inhibited the production of lactonic sophorolipids. However, when organic nitrogen sources were used, lactonic sophorolipid production was strongly increased. Production of crystalline lactonic sophorolipids from organic/inorganic nitrogen sources was enhanced with the increase of pH value adjusted by sodium hydroxide or sodium citrate solution. Fourier-transform infrared (FT-IR), gas chromatography mass spectrometry (GC-MS), high-performance liquid chromatography (HPLC), and mass spectra (MS) were employed to compare the composition of sophorolipid mixture obtained from different nitrogen sources. More than 15 acidic sophorolipid molecules and only 4 lactonic sophorolipid molecules were produced by using 1.27 g/l ammonium sulfate as nitrogen source; they were separated by preparative HPLC and their structures were elucidated by MS. These results suggest extraordinary regulatory effects of nitrogen source on growth and sophorolipid synthesis of W. domercqiae var. sophorolipid.

Keywords

Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 Lactonic sophorolipid Acidic sophorolipid Organic nitrogen source Inorganic nitrogen source 

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Xiao-jing Ma
    • 1
  • Hui Li
    • 1
  • Ling-jian Shao
    • 1
  • Jing Shen
    • 1
  • Xin Song
    • 1
    • 2
  1. 1.State Key Laboratory of Microbial TechnologyShandong UniversityJinanChina
  2. 2.State Key Laboratory of Microbial Technology and National Glycoengineering Research CentreShandong UniversityJinanChina

Personalised recommendations